Martha L Ray

US Patent:

20010055789, Dec 27, 2001

Filed:

Feb 9, 2001

Appl. No.:

09/780878

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

Assignee:

Unigene Laboratories, Inc.

International Classification:

C12P021/02
C12P021/06
C12N001/21

US Classification:

435/069100, 435/069400, 435/252330

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20020045211, Apr 18, 2002

Filed:

Feb 9, 2001

Appl. No.:

09/780643

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

Assignee:

Unigene Laboratories, Inc.

International Classification:

C07K014/00
C07K001/00
A23J001/00
C12N015/74
C12N015/70
C12N015/63
C12N015/00
A61K038/23
A61K038/00
C07K017/00
C07K016/00
C07K007/00
C07K005/00
C12N001/20
C07H021/04
C12P021/06
C12N015/09
C12P021/04

US Classification:

435/069100, 435/320100, 536/024100, 536/023510, 435/252300, 435/252330, 435/069400, 530/307000, 530/417000, 530/416000, 435/069700

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20030118610, Jun 26, 2003

Filed:

Nov 29, 2001

Appl. No.:

09/997465

Inventors:

William Stern - Tenafly NJ, US
Nozer Mehta - Randolph NJ, US
Martha Ray - Nutley NJ, US

Assignee:

Unigene Laboratories Inc.

International Classification:

C12Q001/70
A61K039/12
A61K039/21

US Classification:

424/208100, 424/204100, 435/005000, 424/186100

Abstract:

Bioavailability of peptide active agents to be administered orally is enhanced by a pharmaceutical composition providing targeted release of the peptide to the intestine in addition to having the active peptide linked to a membrane translocator which is capable of being at least partially cleaved in vivo by an enzyme. The composition includes an acid-resistant protective vehicle which transports components of the invention through the stomach and a sufficient amount of a pH-lowering agent to lower local intestinal pH. All components are released together into the intestine with the peptide.

US Patent:

20040191865, Sep 30, 2004

Filed:

Apr 5, 2004

Appl. No.:

10/818966

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

Assignee:

Unigene Laboratories, Inc.

International Classification:

C12P021/02
C12N005/06

US Classification:

435/069100, 435/320100, 435/325000

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with novel vectors, a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special vectors are provided which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20050221442, Oct 6, 2005

Filed:

Mar 9, 2005

Appl. No.:

11/076260

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

International Classification:

C12N015/74
C12N001/20

US Classification:

435069400, 435252330, 435488000

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20080057566, Mar 6, 2008

Filed:

Oct 30, 2007

Appl. No.:

11/928936

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

International Classification:

C12N 5/02

US Classification:

435252330

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20080102491, May 1, 2008

Filed:

Oct 30, 2007

Appl. No.:

11/928994

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

International Classification:

C12P 21/04
C12N 1/20

US Classification:

435069100, 435252330

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.

US Patent:

20080102509, May 1, 2008

Filed:

Oct 30, 2007

Appl. No.:

11/929028

Inventors:

Nozer Mehta - Randolph NJ, US
Angelo Consalvo - Monroe NY, US
Martha Ray - Nutley NJ, US
Christopher Meenan - Lincoln Park NJ, US

International Classification:

C12N 1/20

US Classification:

435252300

Abstract:

Expression systems are disclosed for the direct expression of peptide products into the culture media where genetically engineered host cells are grown. High yield was achieved with a special selection of hosts, and/or fermentation processes which include careful control of cell growth rate, and use of an inducer during growth phase. Special universal cloning vectors are provided for the preparation of expression vectors which include control regions having multiple promoters linked operably with coding regions encoding a signal peptide upstream from a coding region encoding the peptide of interest. Multiple transcription cassettes are also used to increase yield. The production of amidated peptides using the expression systems is also disclosed.