Martin B Lock

US Patent:

20090275107, Nov 5, 2009

Filed:

Apr 27, 2007

Appl. No.:

12/226588

Inventors:

Martin Lock - Southampton PA, US
Luc H. Vandenberghe - Philadelphia PA, US
James M. Wilson - Gladwyne PA, US

Assignee:

The Trustees of the University of Pennsylvania - Philadelphia PA

International Classification:

C12N 7/02
C12N 7/00

US Classification:

435239, 4352351

Abstract:

A method for producing AAV, without requiring cell lysis, is described. The method involves harvesting AAV from the supernatant. For AAV having capsids with a heparin binding site, the method involves modifying the AAV capsids and/or the culture conditions to ablate the binding between the AAV heparin binding site and the cells, thereby allowing the AAV to pass into the supernatant, i.e., media. Thus, the method of the invention provides supernatant containing high yields of AAV which have a higher degree of purity from cell membranes and intracellular materials, as compared to AAV produced using methods using a cell lysis step.