Mary K Mcbride

US Patent:

8232058, Jul 31, 2012

Filed:

Jan 22, 2007

Appl. No.:

12/161291

Inventors:

Mary McBride - Brentwood CA, US
Thomas Slezak - Livermore CA, US
James M. Birch - Albany CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/04

US Classification:

435 612, 435 912, 536 2433

Abstract:

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e. g. , polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

US Patent:

20060057599, Mar 16, 2006

Filed:

Nov 29, 2004

Appl. No.:

11/000491

Inventors:

John Dzenitis - Danville CA, US
Steve Brown - Livermore CA, US
Billy Colston - San Ramon CA, US
Dora Gutierrez - Livermore CA, US
Bruce Henderer - Livermore CA, US
Benjamin Hindson - Belmont, AU
Ramakrishna Madabhushi - Fremont CA, US
Anthony Makarewicz - Livermore CA, US
Don Masquelier - Tracy CA, US
Mary McBride - Brentwood CA, US
Thomas Metz - Tracy CA, US
Shanavaz Nasarabadi - Livermore CA, US
Ujwal Setlur - Livermore CA, US
Sally Smith - Salida CA, US
Kodumudi Venkateswaran - Livermore CA, US

International Classification:

C12Q 1/68
C12M 1/34

US Classification:

435006000, 435030000, 435287200, 435288500

Abstract:

An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for detecting said bioagents.

US Patent:

20070166725, Jul 19, 2007

Filed:

Jan 18, 2006

Appl. No.:

11/335074

Inventors:

Mary McBride - Brentwood CA, US
Benjamin Hindson - Livermore CA, US
Steve Brown - Livermore CA, US

International Classification:

C12Q 1/68
C12M 3/00

US Classification:

435006000, 435287200

Abstract:

The invention provides a system for high-throughput multiplex analysis of target samples. A sample and reagent delivery unit is operatively connected to a thermal cycler for amplification of target nucleic acids. Microspheres are hybridized to the resulting amplicons in the thermal cycler. A flow cytometer is operatively connected to the thermal cycler or optionally a bead trap for washing the microspheres.

US Patent:

20070172835, Jul 26, 2007

Filed:

Jan 20, 2006

Appl. No.:

11/335977

Inventors:

Mary McBride - Brentwood CA, US
Thomas Slezak - Livermore CA, US
James Birch - Albany CA, US

International Classification:

C12Q 1/68
C07H 21/04
C12M 1/34

US Classification:

435006000, 536024300, 435287200, 977924000

Abstract:

The present invention is directed to methods, compositions and kits for multiplex detection of pathogens, such as respiratory pathogens.

US Patent:

20080248459, Oct 9, 2008

Filed:

Jan 12, 2007

Appl. No.:

11/652841

Inventors:

Mary Teresa McBride - Brentwood CA, US
Thomas Richard Slezak - Livermore CA, US
Sharon Lee Messenger - Kensington CA, US

International Classification:

C12Q 1/70

US Classification:

435 5

Abstract:

Described are kits and methods useful for detection of seven agricultural pathogens (BPSV; BHV; BVD; FMDV; BTV; SVD; and VESV) in a sample. Genomic sequence information from 7 agricultural pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.