Matthew P Coleman

US Patent:

7585815, Sep 8, 2009

Filed:

Jul 1, 2004

Appl. No.:

10/884783

Inventors:

Peter T. Beernink - Walnut Creek CA, US
Matthew A. Coleman - Oakland CA, US
Brent W. Segelke - San Ramon CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

C40B 20/02
C40B 20/04
A23J 16/00
C12Q 1/68
C12P 21/06

US Classification:

506 3, 506 4, 435 6, 435 681, 530412

Abstract:

Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.

US Patent:

7901623, Mar 8, 2011

Filed:

Sep 26, 2006

Appl. No.:

11/528286

Inventors:

Robin R. Miles - Danville CA, US
William J. Benett - Livermore CA, US
Matthew A. Coleman - Oakland CA, US
Francesca S. Pearson - Livermore CA, US
Shanavaz L. Nasarabadi - Livermore CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01N 33/00

US Classification:

422 58, 436164, 436169, 435 4, 4352871, 422 50, 422 55, 422 61, 422 681, 422104

Abstract:

A lateral flow strip assay apparatus comprising a housing; a lateral flow strip in the housing, the lateral flow strip having a receiving portion; a sample collection unit; and a reagent reservoir. Saliva and/or buccal cells are collected from an individual using the sample collection unit. The sample collection unit is immersed in the reagent reservoir. The tip of the lateral flow strip is immersed in the reservoir and the reagent/sample mixture wicks up into the lateral flow strip to perform the assay.

US Patent:

8428398, Apr 23, 2013

Filed:

Jun 29, 2010

Appl. No.:

12/826215

Inventors:

Deanna Lynn Thompson - Orangevale CA, US
Matthew A. Coleman - Oakland CA, US
Stephen M. Lane - Oakland CA, US
Dennis L. Matthews - Gold River CA, US
Joanna Albala - Stockton CA, US
Sebastian Wachsmann-Hogiu - Sacramento CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA
The Regents of the University of California - Oakland CA

International Classification:

G06K 9/22

US Classification:

382313, 348294

Abstract:

A hand-held portable microarray reader for biodetection includes a microarray reader engineered to be small enough for portable applications. The invention includes a high-powered light-emitting diode that emits excitation light, an excitation filter positioned to receive the excitation light, a slide, a slide holder assembly for positioning the slide to receive the excitation light from the excitation filter, an emission filter positioned to receive the excitation light from the slide, a lens positioned to receive the excitation light from the emission filter, and a CCD camera positioned to receive the excitation light from the lens.

US Patent:

7993583, Aug 9, 2011

Filed:

Oct 6, 2009

Appl. No.:

12/574095

Inventors:

Lawrence Christopher Dugan - Modesto CA, US
Matthew A. Coleman - Oakland CA, US

Assignee:

Lawrence Livermore National Security, LLC - Livermore CA

International Classification:

G01N 21/00
G01N 33/543
C12M 3/00
G01N 33/53
C40B 60/12

US Classification:

422 64, 422 63, 422 65, 422 681, 422 8205, 435 6, 435 71, 4352872, 4352873, 4352887, 436164, 436518, 436527, 506 39

Abstract:

A microfluidic array card and reader system for analyzing a sample. The microfluidic array card includes a sample loading section for loading the sample onto the microfluidic array card, a multiplicity of array windows, and a transport section or sections for transporting the sample from the sample loading section to the array windows. The microfluidic array card reader includes a housing, a receiving section for receiving the microfluidic array card, a viewing section, and a light source that directs light to the array window of the microfluidic array card and to the viewing section.

US Patent:

20060234272, Oct 19, 2006

Filed:

Mar 27, 2006

Appl. No.:

11/390584

Inventors:

Andrew Wyrobek - Walnut Creek CA, US
Matthew Coleman - Oakland CA, US
David Nelson - Oakland CA, US

International Classification:

C12Q 1/68

US Classification:

435006000

Abstract:

A method of predicting sensitivity to radiation comprises selecting at least one of a first panel of genes associated with increased chromosomal damage and a second panel of genes associated with reduced chromosomal damage. The tissue is exposed to radiation. The RNA of the tissue is measured providing measured RNA of the first panel of genes associated with increased chromosomal damage and providing measured RNA of the second panel of genes associated with reduced chromosomal damage. Sensitivity to radiation is predicted using at least one of the measured RNA of the first panel of genes associated with increased chromosomal damage and the measured RNA of the second panel of genes associated with reduced chromosomal damage.

US Patent:

20080076122, Mar 27, 2008

Filed:

Sep 26, 2006

Appl. No.:

11/528296

Inventors:

Andrew J. Wyrobek - Walnut Creek CA, US
Matthew A. Coleman - Oakland CA, US
David O. Nelson - Oakland CA, US
James Tucker - Novi MI, US

International Classification:

C12Q 1/68
C12P 19/34
G06F 19/00

US Classification:

435 6, 435 912, 702 20

Abstract:

A method of characterizing exposure to ionizing radiation, utilizing the steps of selecting a set of biomarker geness for characterizing exposure to ionizing radiation, and using the set of biomarker genes for characterizing exposure to ionizing radiation. The step of selecting a set of biomarker genes for characterizing exposure to ionizing radiation was developed utilizing a unique set of 420 oligonucleotide probes for human genes capable of discerning past exposure to different doses of ionizing radiation. The step of selecting a set of biomarker genes for characterizing exposure to ionizing radiation utilizes groupings of genes that represent candidate panels of mRNA biomarkers.

US Patent:

20080200342, Aug 21, 2008

Filed:

Feb 14, 2008

Appl. No.:

12/031625

Inventors:

Rupa S. Rao - Stockton CA, US
Stephen M. Lane - Oakland CA, US
Dennis L. Matthews - Moss Beach CA, US
Matthew A. Coleman - Oakland CA, US

International Classification:

C40B 30/04
C40B 60/12
C40B 30/00

US Classification:

506 9, 506 39, 506 7

Abstract:

A device and array coupled to capture molecules are provided. Specifically, the device and array can be used for detecting the presence and concentration of biomarkers in a sample from a subject. The device and array can also allow the use of a method for scoring a sample for, e.g., the purpose of diagnosing a disease. The method can also be advantageous to applications where there is a need to accurately determine the disease stage of a subject for the purpose of making therapeutic decisions.

US Patent:

20090136937, May 28, 2009

Filed:

May 9, 2008

Appl. No.:

12/118530

Inventors:

Matthew A. COLEMAN - Oakland CA, US
Paul D. Hoeprich - Pleasanton CA, US
Erin S. Arroyo - Fairfax CA, US
Jenny A. Cappuccio - Livermore CA, US

International Classification:

C12Q 1/68
G01N 33/68
G01N 33/566
C12M 1/00
B01J 19/00
G01N 33/53

US Classification:

435 6, 436 86, 436501, 435 79, 422 681, 4352871, 4352872

Abstract:

Provided herein are methods and systems for the monitoring production of a target protein in of a nanolipoprotein particle (NLP) that also includes a scaffold protein and a membrane forming lipid. The target protein is capable of assuming an active form and an inactive form. Monitoring is performed by an indicator protein that is capable of assuming an active form and an inactive form, the active form associated with a detectable activity of the indicator protein, the detectable activity further associated with the active form of the target protein.