Matthew Carl Peters

US Patent:

7524664, Apr 28, 2009

Filed:

Jun 15, 2004

Appl. No.:

10/869825

Inventors:

Frances H. Arnold - La Cañada CA, US
Matthew W. Peters - Pasadena CA, US
Peter Meinhold - Pasadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C12N 9/02
C12N 15/00
C12N 1/20
C12P 7/00
C12P 21/04
C07H 21/04
C12Q 1/68
C12Q 1/00
C12Q 1/26

US Classification:

435189, 435 4, 435 6, 435 691, 435 711, 435 25, 435132, 435440, 4352523, 4353201, 536 232

Abstract:

Cytochrome P450 BM-3 from was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with , where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.

US Patent:

7863030, Jan 4, 2011

Filed:

Apr 15, 2009

Appl. No.:

12/424454

Inventors:

Frances H Arnold - La Canada CA, US
Matthew W Peters - Pasadena CA, US
Peter Meinhold - Pasadena CA, US

Assignee:

The California Institute of Technology - Pasadena CA

International Classification:

C12N 9/02
C12N 15/00
C12N 1/20
C12P 7/00
C12P 21/04
C07H 21/04
C12Q 1/68
C12Q 1/00
C12Q 1/26

US Classification:

435189, 435 4, 435 6, 435 691, 435 711, 435 25, 435132, 435440, 4352523, 4353201, 536 232

Abstract:

Cytochrome P450 BM-3 from was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with , where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.

US Patent:

8343744, Jan 1, 2013

Filed:

Jan 3, 2011

Appl. No.:

12/983841

Inventors:

Frances H Arnold - La Canada CA, US
Matthew W Peters - Pasadena CA, US
Peter Meinhold - Pasadena CA, US

Assignee:

The California Institute of Technology - Pasadena CA

International Classification:

C12N 9/02
C12N 15/00
C12P 7/00
C12P 21/06
C12P 21/04
C07H 21/04

US Classification:

435189, 435 711, 435440, 435132, 435 691, 536 232

Abstract:

Cytochrome P450 BM-3 from was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-position to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with , where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.

US Patent:

20080057577, Mar 6, 2008

Filed:

Apr 6, 2007

Appl. No.:

11/697404

Inventors:

Frances Arnold - Pasadena CA, US
Peter Meinhold - Pasadena CA, US
Matthew Peters - Pasadena CA, US
Rudi Fasan - Brea CA, US
Mike Chen - Pasadena CA, US

Assignee:

California Institute of Technology - Pasadena CA

International Classification:

C07K 14/32
C07K 1/107
C12N 1/19
C12N 1/21
C12N 5/10

US Classification:

435348000, 435252300, 435254200, 435325000, 530350000, 530402000, 536023700

Abstract:

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.

US Patent:

20090155869, Jun 18, 2009

Filed:

Dec 3, 2007

Appl. No.:

11/949724

Inventors:

Thomas Buelter - Santa Monica CA, US
Andrew C. Hawkins - Pasadena CA, US
Kalib Kersh - LaVerne CA, US
Peter Meinhold - Pasadena CA, US
Matthew W. Peters - Pasadena CA, US
Ezhilkani Subbian - Pasadena CA, US

Assignee:

Gevo, Inc. - Pasadena CA

International Classification:

C12P 7/16
C12N 1/21

US Classification:

435160, 4352523

Abstract:

A recombinant microorganism expressing at least a heterologous enzyme of an NADH-dependent pathway for conversion of a carbon source to n-butanol, metabolic intermediate and/or a derivative thereof and capable of producing n-butanol, a metabolic intermediate and/or a derivative thereof at a high yield and related methods. The recombinant microorganism engineered to inactivate a native enzyme of one or more pathways that compete with NADH-dependent heterologous pathway, and/or to balance the NADH-dependent heterologous pathway with respect to NADH production and consumption.

US Patent:

20100062505, Mar 11, 2010

Filed:

Dec 21, 2007

Appl. No.:

11/963542

Inventors:

Uvini Gunawardena - Pasadena CA, US
Peter Meinhold - Pasadena CA, US
Matthew W. Peters - Pasadena CA, US
Jun Urano - Culver City CA, US
Reid M. Renny Feldman - San Marino CA, US

Assignee:

Gevo, Inc. - Pasadena CA

International Classification:

C12P 7/16
C12N 1/18
C12N 1/15

US Classification:

435160, 4352542, 43525421

Abstract:

There are disclosed metabolically-engineered yeast and methods of producing n-butanol. In an embodiment, metabolically-engineered yeast is capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway capable of utilizing NADH to convert acetyl-CoA to n-butanol. In another embodiment, a method of producing n-butanol includes (a) providing metabolically-engineered yeast capable of metabolizing a carbon source to produce n-butanol, at least one pathway produces increased cytosolic acetyl-CoA relative to cytosolic acetyl-CoA produced by a wild-type yeast, and at least one heterologous gene encodes and expresses at least one enzyme for a metabolic pathway utilizing NADH to convert acetyl-CoA to n-butanol; and (b) culturing the yeast to produce n-butanol. Other embodiments are also disclosed.

US Patent:

20130273626, Oct 17, 2013

Filed:

Nov 9, 2012

Appl. No.:

13/673977

Inventors:

The California Institute of Technology - , US
Matthew W. Peters - Pasadena CA, US
Peter Meinhold - Pasadena CA, US

International Classification:

C12N 9/00

US Classification:

435157, 435189

Abstract:

Cytochrome P450 BM-3 from was engineered using a combination of directed evolution and site-directed mutagenesis to hydroxylate linear alkanes regio- and enantioselectively using atmospheric dioxygen as an oxidant. Mutant 9-10A-A328V hydroxylates octane primarily at the 2-positio to form S-2-octanol (40% ee). Another mutant, 1-12G, hydroxylates alkanes larger than hexane primarily at the 2-position, but forms R-2-alcohols (40-55% ee). These biocatalysts are highly active for alkane substrates and support thousands of product turnovers. These regio- and enantio-selectivities are retained in whole-cell biotransformations with , where the engineered P450s can be expressed at high levels and the expensive cofactor is supplied endogenously.

US Patent:

20200399662, Dec 24, 2020

Filed:

May 11, 2020

Appl. No.:

16/872275

Inventors:

- Pasadena CA, US
Peter Meinhold - Pasadena CA, US
Matthew W. Peters - Pasadena CA, US
Rudi Fasan - Brea CA, US
Mike M.Y. Chen - Pasadena CA, US

International Classification:

C12P 7/04
C07K 14/32
C12N 9/02
C12P 7/06

Abstract:

This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells.