Matthew P Peters

US Patent:

20110287500, Nov 24, 2011

Filed:

Jul 5, 2011

Appl. No.:

13/176452

Inventors:

Jun URANO - Irvine CA, US
Catherine Asleson Dundon - Englewood CO, US
Peter Meinhold - Denver CO, US
Reid M. Renny Feldman - San Francisco CA, US
Aristos Aristidou - Highlands Ranch CO, US
Andrew Hawkins - Parker CO, US
Thomas Buelter - Denver CO, US
Matthew Peters - Highlands Ranch CO, US
Doug Lies - Parker CO, US
Ruth Berry - Englewood CO, US
Ishmeet Kalra - Englewood CO, US

Assignee:

GEVO, INC. - Englewood CO

International Classification:

C12P 7/16
C12N 1/19

US Classification:

435160, 4352542, 43525421

Abstract:

The present invention provides recombinant microorganisms comprising isobutanol producing metabolic pathway with at least one isobutanol pathway enzyme localized in the cytosol, wherein said recombinant microorganism is selected to produce isobutanol from a carbon source. Methods of using said recombinant microorganisms to produce isobutanol are also provided. In various aspects of the invention, the recombinant microorganisms may comprise a cytosolically active isobutanol pathway enzymes. In some embodiments, the invention provides mutated, modified, and/or chimeric isobutanol pathway enzymes with cytosolic activity. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.

US Patent:

20120288910, Nov 15, 2012

Filed:

Jun 20, 2012

Appl. No.:

13/528106

Inventors:

Jun Urano - Irvine CA, US
Catherine Asleson Dundon - Englewood CO, US
Peter Meinhold - Denver CO, US
Reid M. Renny Feldman - San Francisco CA, US
Aristos Aristidou - Englewood CO, US
Andrew Hawkins - Parker CO, US
Thomas Buelter - Denver CO, US
Matthew Peters - Highlands Ranch CO, US
Doug Lies - Parker CO, US
Christopher Smith - Englewood CO, US
Lynne H. Albert - Englewood CO, US

Assignee:

Gevo, Inc. - Englewood CO

International Classification:

C12P 7/16
C12N 1/19

US Classification:

435160, 4352542, 43525421, 43525423, 43525422

Abstract:

The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the , Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.