Melissa J Moore

US Patent:

20220298516, Sep 22, 2022

Filed:

Sep 20, 2019

Appl. No.:

17/276983

Inventors:

- Cambridge MA, US
Jennifer NELSON - Brookline MA, US
Melissa MOORE - Cambridge MA, US

International Classification:

C12N 15/67
C12N 15/88
A61K 47/00
A61K 31/7088
C12N 15/11

Abstract:

The present disclosure relates to methods and compositions for modulating protein expression. In particular, the invention features methods and compositions for increasing protein expression in a cell by delivering to the cell a composition including an mRNA encoding a polypeptide and one or more oligonucleotides, wherein each of the one or more oligonucleotides includes a region of linked nucleotides complimentary to a portion of the sequence of the mRNA. The methods and compositions described herein may be used to modulate gene expression (e.g., increase gene expression), to increase the stability of the mRNA, to decrease the immunogenicity of the mRNA, to enable selective expression (e.g., in a target cell or tissue) of the mRNA, and/or to enable the delivery of two or more mRNAs in a stoichiometric ratio.

US Patent:

20210378980, Dec 9, 2021

Filed:

Sep 20, 2019

Appl. No.:

17/277994

Inventors:

- Cambridge MA, US
Christopher Karl MCLAUGHLIN - Cambridge MA, US
Jessica CHENEY - Holliston MA, US
Ben GELDHOF - Cambridge MA, US
Jeffrey HRKACH - Lexington MA, US
Melissa J. MOORE - Chestnut Hill MA, US
Stephen G. HOGE - Brookline MA, US

International Classification:

A61K 9/51
A61K 45/06

Abstract:

The present disclosure provides methods of producing lipid nanoparticle (LNP) formulations and the produced LNP formulations thereof. The present disclosure also provides therapeutic and diagnostic uses related to the produced LNP formulations.

US Patent:

20210163928, Jun 3, 2021

Filed:

Apr 11, 2019

Appl. No.:

17/041332

Inventors:

- Cambridge MA, US
Caroline KÖHRER - Cambridge MA, US
Ruchi JAIN - Brookline MA, US
Melissa J. MOORE - Cambridge MA, US
Scott DONOVAN - Braintree MA, US
Aaron LARSEN - Cambridge MA, US
Vladimir PRESNYAK - Manchester NH, US

International Classification:

C12N 15/11
A61K 48/00
C12Q 1/6897

Abstract:

The present disclosure provides messenger RNAs (mRNAs) having chemical and/or structural modifications, including RNA elements and/or modified nucleotides, in particular C-rich or CG-rich elements, which provide a desired translational regulatory activity to the mRNA.

US Patent:

20200362382, Nov 19, 2020

Filed:

Aug 17, 2018

Appl. No.:

16/639881

Inventors:

- Cambridge MA, US
Aaron LARSEN - Boston MA, US
I-Ping CHAN - Newton MA, US
Jennifer NELSON - Brookline MA, US
William ISSA - Dedham MA, US
Melissa J. MOORE - Chestnut Hill MA, US

International Classification:

C12P 19/34
C07H 21/02

Abstract:

The present disclosure relates to methods for preparing a modified RNA comprising enzymatically ligating an acceptor moiety having a 3′-hydroxyl group to the 5′-end of a donor moiety (e.g., installation of 5′-triphosphate groups, mRNA caps, or cap-like structures on chemically synthesized RNA or installation of 5′-triphosphate groups, mRNA caps, cap-like structures, or non-cap like structures on enzymatically prepared RNA).

US Patent:

20160289734, Oct 6, 2016

Filed:

Apr 1, 2016

Appl. No.:

15/089243

Inventors:

- Boston MA, US
Melissa Jeanne Moore - Chestnut Hill MA, US
Samson Michael Jolly - Holden MA, US
William Edward Salomon - Worcester MA, US
Victor Serebrov - Shrewsbury MA, US
Han Zhang - Zhengzhou, CN

International Classification:

C12Q 1/68
G01N 33/53

Abstract:

The invention relates to the use of Argonaute polypeptide:guide molecule complexes as fast and specific nucleic acid probes, as specific, nucleic acid-guided restriction enzymes for DNA and RNA substrates, and as a means to detect RNA-protein interactions, RNA detection, DNA detection, and RNA depletion. Using such Argonaute polypeptide:guide molecule complexes enables fast and specific detection, purification, and enzymatic activity.

US Patent:

20150240232, Aug 27, 2015

Filed:

Jan 22, 2015

Appl. No.:

14/602756

Inventors:

- Boston MA, US
Melissa J. Moore - Chestnut Hill MA, US
Christian Roy - Shrewsbury MA, US

International Classification:

C12N 15/10

Abstract:

A technology is described that is capable of generating high-throughput sequencing (HTS) read length DNA products to accurately and reliably provide exon connectivity information for alternatively spliced isoforms. The method is not limited by the initial size of the isoform as the technology removes the template oligonucleotide sequence and a newly formed full length ligated product provides an HTS-compatible read length sequence that comprises information that corresponds to the consecutive order of the exons in the original template oligonucleotide.