Melvin Kim Wong

US Patent:

51151014, May 19, 1992

Filed:

Jun 26, 1991

Appl. No.:

7/721005

Inventors:

James W. Bloom - Richmond CA
Melvin F. Wong - San Francisco CA
Gautam Mitra - Kensington CA

Assignee:

Miles Inc. - Elkhart IN

International Classification:

C07K 322
C07K 1528
C07K 1500

US Classification:

53038825

Abstract:

Antibody preparation purified using immobilized protein A and yet substantially free of protein A that may have solubilized during the purificaiton process. The antibodies include less than 15 ng protein A per mg of antibody, preferably less than 1 ng/mg. Low protein A content is obtained by first contacting the antibodies and solubilized protein A with an ion exchange resin under conditions to adsorb both. The antibodies and protein A are then sequentially eluted under conditions of increasing ionic strength.

US Patent:

49837222, Jan 8, 1991

Filed:

Jun 8, 1988

Appl. No.:

7/204054

Inventors:

James W. Bloom - Richmond CA
Melvin F. Wong - San Francisco CA
Gautam Mitra - Kensington CA

Assignee:

Miles Inc. - Elkhart IN

International Classification:

C07K 322
C07K 318

US Classification:

530387

Abstract:

Protein A is selectively isolated from an antibody--Protein A mixture by exposing the mixture to an anion exchange material under conditions sufficient to adsorb both components and then sequentially eluting the antibodies and protein A under conditions of increasing ionic strength. Resulting antibody preparations have less than about 15 ng of Protein A per mg of antibody.