Meredith A Jones

US Patent:

20130316400, Nov 28, 2013

Filed:

May 2, 2013

Appl. No.:

13/886226

Inventors:

Henry CHIOU - Encinitas CA, US
Jeffrey ROGERS - Escondido CA, US
Maria Isabel CISNEROS - San Marcos CA, US
Jingqiu LI - San Diego CA, US
Chao Yan LIU - Clarence Center NY, US
Meredith JONES - Chapel Hill NC, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C07K 14/505

US Classification:

435 691

Abstract:

The present invention is directed generally to cell culture media (particularly serum free, non animal derived, and/or chemically defined media) which are useful for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). According to the invention, such introduction can take place in the presence of said medium. Cells containing such introduced materials can then be cultured in the medium and the effect of the introduced materials on the cells can be measured or determined. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly eukaryotic cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

US Patent:

20230130038, Apr 27, 2023

Filed:

Oct 31, 2022

Appl. No.:

17/977788

Inventors:

- Carlsbad CA, US
Chao Yan LIU - Germantown MD, US
Jeffrey ROGERS - San Diego CA, US
Maria Isabel CISNEROS - San Diego CA, US
Jingqui LI - San Diego CA, US
Henry C. CHIOU - Encinitas CA, US
Meredith Bauman JONES - Chapel Hill NC, US

International Classification:

C07K 14/505
C12N 5/00
C12N 5/0793

Abstract:

High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.

US Patent:

20190077841, Mar 14, 2019

Filed:

Jul 26, 2018

Appl. No.:

16/046641

Inventors:

- Carlsbad CA, US
Chao Yan LIU - Germantown MD, US
Jeffrey ROGERS - San Diego CA, US
Maria CISNEROS - San Diego CA, US
Jingqiu LI - San Diego CA, US
Henry CHIOU - Encinitas CA, US
Meredith JONES - Chapel Hill NC, US

International Classification:

C07K 14/505
C12N 5/0793
C12N 5/00

Abstract:

High-yield mammalian transient expression systems can include a cell culture media (particularly serum free, non-animal derived, and/or chemically defined media) for introducing macromolecules and compounds (e.g., nucleic acid molecules) into cells (e.g., eukaryotic cells). Cells containing such introduced materials can then be cultured in the cell culture media. In particular, the invention allows introduction of nucleic acid molecules (e.g., vectors) into cells (particularly mammalian cells) and expression of proteins encoded by the nucleic acid molecules in the cells. The invention obviates the need to change the cell culture medium each time a different procedure is performed with the cells (e.g., culturing cells vs. transfecting cells). The invention also relates to compositions and kits useful for culturing and transforming/transfecting cells.