Meredith J Wright

US Patent:

55592074, Sep 24, 1996

Filed:

Apr 14, 1994

Appl. No.:

8/227370

Inventors:

Jonathan L. Sessler - Austin TX
Daniel A. Smith - Goshen IN
Richard A. Miller - Portola Valley CA
Kevin L. Ross - Blue Ash OH
Meredith Wright - San Jose CA
William C. Dow - Fremont CA
Vladimir A. Kr al - Austin TX
Brent Iverson - Austin TX
Darren Magda - Cupertino CA

Assignee:

Board of Regents, University of Texas - Austin TX
Pharmacyclics, Inc. - Sunnyvale CA

International Classification:

C07K 200
C07K 1600
C07H 300
C07F 500

US Classification:

530300

Abstract:

A method of phosphate ester hydrolysis including incubating a solution of an aqueous phosphate ester with a texaphyrin metal complex. The metal is a metal cation having catalytic activity for ester bond hydrolysis in aqueous solution, in particular, a lanthanide metal cation, preferably Eu(III) or Dy(III). A preferred substrate is RNA and a preferred texaphyrin is a derivatized texaphyrin having binding specificity, in particular, a texaphyrin covalently coupled to a site-directed molecule, preferably an oligonucleotide.