Michael T. Boenisch - Owings Mills MD Erica A. Sullivan - Sparks MD
Assignee:
Becton, Dickinson and Company - Franklin Lakes NJ
International Classification:
G01N 33566 C12N 996
US Classification:
435 78
Abstract:
This invention presents a non-isotopic competitive assay for Vitamin B12 (B12), utilizing intrinsic factor (IF) labelled with horse radish peroxidase (HRP), by coupling via heterobifunctional cross-linking agents. In addition, a method for stabilizing the resultant conjugates by pretreatment with N-ethylmaleimide is disclosed.