Michael A Buckholt

US Patent:

57120919, Jan 27, 1998

Filed:

Mar 29, 1994

Appl. No.:

8/219746

Inventors:

Joseph C. Bagshaw - Holden MA
Michael A. Buckholt - Worcester MA

Assignee:

Worcester Polytechnic Institute - Worcester MA

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

A method for the selection of shrimp having a genetically-transmitted favorable growth characteristic for improved aquacultured shrimp production. A genetic marker for the favorable characteristic is prepared by isolating Penaeus shrimp nuclear DNA by extraction with chloroform in the presence of cetyl trimethyl ammonium bromide, digesting the DNA with one or more restriction enzymes, and identifying characteristic restriction fragments. The marker is hybridized to a nuclear nucleic acid molecule isolated from Penaeus shrimp for the selection of shrimp having the desired characteristic. The marker can be a labelled probe or a primer for amplification and subsequent detection of a gene that encodes a protein promoting the desired growth characteristic. The selected shrimp can be used to produce a high quality, genetically superior seedstock or larvae useful for the economic production of aquacultured shrimp.