Michael Aaron Cantrell

US Patent:

48822825, Nov 21, 1989

Filed:

Jul 31, 1986

Appl. No.:

6/888994

Inventors:

Dirk M. Anderson - Seattle WA
Paul E. Baker - Bainbridge Island WA
Michael A. Cantrell - Seattle WA
Douglas P. Cerretti - Seattle WA
David J. Cosman - Seattle WA
Steven D. Gimpel - Seattle WA
Kenneth H. Grabstein - Seattle WA
Alf D. Larsen - Seattle WA
Kate N. McKereghan - Seattle WA

Assignee:

Immunex Corporation - Seattle WA

International Classification:

C12N 100
C12N 1500
C12P 200
C12P 1734

US Classification:

4352523

Abstract:

A chemically-synthesized oligonucleotide composing a portion of the nucleotide sequence of the human IL-2 is employed as a probe to isolate the gene coding for human IL-2. The human IL-2 gene is selected from a cDNA library prepared from RNA produced by mitogen-stimulated Jurkat cells. Double-stranded cDNA is prepared from polyadenylated RNA extracted from bovine cells thought to produce interleukin-2. Such cDNA is inserted within a plasmid vector and the recombinant plasmid employed to transform hosts. Plasmid DNA, prepared from pools of the transformed hosts, is hybridized with a probe composed of a large portion of the coding sequence of the human IL-2 gene. Pools of host cells that provide signal to the human cDNA probe are identified, subdivided, and rescreened until a single positive colony is identified. Bovine plasmid cDNA is prepared from this colony, and the bIL-2 gene is sequenced. The plasmid DNA is employed to express recombinant bovine IL-2 in yeast and bacterial expression systems, with the expressed bovine IL-2 purified to homogeneity by one or more reverse phase high-performance liquid chromatography procedures.

US Patent:

50064657, Apr 9, 1991

Filed:

Aug 11, 1989

Appl. No.:

7/392811

Inventors:

Dirk M. Anderson - Seattle WA
Paul E. Baker - Bainbridge Island WA
Michael A. Cantrell - Seattle WA
Douglas P. Cerretti - Seattle WA
David J. Cosman - Seattle WA
Steven D. Gimpel - Seattle WA
Kenneth H. Grabstein - Seattle WA
Alf D. Larsen - Seattle WA
Kate N. McKereghan - Seattle WA

International Classification:

C12P 2106
C12P 1934
C12N 1500
C12N 120
C12N 114
C12N 118
C12N 1500
C07H 1512
C07K 300

US Classification:

435 6952

Abstract:

A process for preparing bovine interleukin-2 (bIL-2) by culturing a microbial host transformed by a vector containing a DNA sequence encoding bIL-2 under conditions suitable for the expression of bIL-2 and recovering bIL-2.

US Patent:

58342869, Nov 10, 1998

Filed:

May 24, 1995

Appl. No.:

8/374652

Inventors:

Helena K. M. Nevalainen - North Epping, AU
Marja T. Paloheimo - Helsinki, FI
Richard B. Fagerstrom - Espoo, FI
Arja S. K. Miettinen-Oinonen - Masala, FI
Marja K. Turunen - Helsinki, FI
John A. Rambosek - Seattle WA
Christopher S. Piddington - Seattle WA
Christine S. Houston - Bothell WA
Michael A. Cantrell - Moscow ID

Assignee:

Rohm Enzyme Finland Oy - Rajamaki

International Classification:

C12N 115
C12N 121
C12N 916
C12S 300

US Classification:

435196

Abstract:

The present invention provides a recombinant combination strain which is capable of over-expressing at least two different genes under two separate promoters in filamentous fungi. The genes encode phytase and pH 2. 5 acid phosphatase. Mixtures containing desired ratios of the two enzymes are prepared by recombinant DNA techniques. The enzyme mixtures show a cooperative effect in the degradation of phytic acid and its salts. The preferred ratios of the two enzymes are from about 3:1 to about 16:1.

US Patent:

57802920, Jul 14, 1998

Filed:

Jul 31, 1992

Appl. No.:

7/923724

Inventors:

Helena K. M. Nevalainen - Espoo, FI
Marja T. Paloheimo - Helsinki, FI
Aria S. K. Miettinen-Oinonen - Masala, FI
Tuula K. Torkkeli - Helsinki, FI
Michael Cantrell - Seattle WA
Cristopher S. Piddington - Seattle WA
John A. Rambosek - Seattle WA
Marja K. Turunen - Helsinki, FI
Richard B. Fagerstrom - Espoo, FI

Assignee:

Alko Group Ltd. - Helsinki

International Classification:

C12N 114
C12N 115
C12N 916
A23K 100

US Classification:

4352568

Abstract:

A highly efficient overexpression system for phytase and pH 2. 5 acid phosphatase in Trichoderma is described. This system results in enzyme compositions that are especially useful in the animal feed industry.