Michael Leuck

US Patent:

7427678, Sep 23, 2008

Filed:

May 1, 2001

Appl. No.:

09/845742

Inventors:

Wolfgang Pieken - Boulder CO, US
Andreas Wolter - Hamburg, DE
David P. Sebesta - Longmont CO, US
Michael Leuck - Boulder CO, US
John Pilon - Ft. Collins CO, US
Gregory M. Husar - Longmont CO, US

Assignee:

Sigma-Aldrich Co. - St. Louis MO

International Classification:

C07H 21/00
A61K 38/00
C07K 1/00

US Classification:

536 253, 536 2534, 530338

Abstract:

This invention discloses a novel method for immobilizing molecules to a support. Specifically, this invention discloses a method of immobilizing derivatized biomolecules, such as oligonucleotides, using cycloaddition reactions, such as the Diels-Alder reaction. Included in this invention are the novel immobilized biomolecules that can be prepared according to the method of this invention.

US Patent:

20030195351, Oct 16, 2003

Filed:

Jan 22, 2003

Appl. No.:

10/349195

Inventors:

Wolfgang Pieken - Boulder CO, US
Andreas Wolter - Hamburg, DE
Michael Leuck - Boulder CO, US

Assignee:

PROLIGO, LLC - Boulder CO

International Classification:

C07H021/04

US Classification:

536/025300

Abstract:

The present invention discloses novel methods for the integrated synthesis and purification of oligonucleotides. The methods employ novel capping reagents carrying two functional groups. The first functional group provides for a smooth and efficient capping process and incorporates the second functional group into contaminant oligonucleotides during solid phase oligonucleotide synthesis. The second functional group functions as a chemical purification handle in the trapping of truncated oligonucleotides (failure sequences) on a solid support. The trapping process creates covalent bonds between the solid support and the truncated oligonucleotides and therefore allows the removal of the truncated sequences from the desired full length oligonucleotide product by filtration. The chemical trapping process employed in this invention is based on cycloaddition reactions, particularly Diels-Alder reactions between the truncated oligonucleotides and the trapping agent. The invention includes novel solid support compositions that carry covalently attached Diels-Alder reaction components.