525 Washington Blvd, Jersey City, NJ 07310 525 Washington Blvd 16 Flr STE 1660, Jersey City, NJ 07310 99 Wood Ave S, Iselin, NJ 08830 (732)6353899, (201)2160777, (732)6353874
Michael McClelland - New York NY Louis G. Kessler - Dallas TX
Assignee:
University of Georgia Research Center - Athens GA
International Classification:
C12P 1934 C12P 2100 C07H 1512 C12Q 160
US Classification:
435 91
Abstract:
The present invention provides a method for site-specific cleavage of double stranded DNA at sequences not less than eight base pairs long, comprising methylating he DNA with a sequence-specific methylase capable of recognizing and methylating a first sequence of the DNA, thereby generating a second sequence of the DNA capable of being recognized by a site-specific endonuclease, the first and second sequences having an overlapping part thereof; the length of the combined methylase and endonuclease recognition sites being not less than eight base pairs long, and cleaving the methylated DNA by treatment with the site-specific endonuclease. This method is useful for increasing the selectivity of cutting DNA stands using restriction endonucleases, thereby permitting the isolation of large DNA fragments and the generation of unique sites in DNA fragments, e. g. , cloning vectors. Also provided is a DNA vector having a terminal sequence not less than eight base pairs long which can be recognized and cut by a site-specific endonuclease.
Resumes
Engineering Technician Supervisor At Caci International Inc
Engineering Technician Supervisor at CACI International Inc
Location:
Springfield, Virginia
Industry:
Information Technology and Services
Work:
CACI International Inc since Jan 2010
Engineering Technician Supervisor
United States Army May 2003 - Jul 2009
Integrated Electronics Warfare Technician
Video Production Services Jul 2000 - May 2003
Chief Television Engineer
Video Production Services Oct 1998 - Jul 2000
Television Engineer
MICHAEL MCCLELLAND Apr 1994 - Oct 1998
Systems Methods Technician
Education:
ECPI College of Technology 1997 - 1998
Associate of Applied Science Degree, Electronics Technology
U.S. Army
Assistant Vice President - Operations Manager at Financial Services
Location:
Tyler, Texas
Industry:
Financial Services
Work:
Financial Services - Tyler, TX since Nov 2009
Assistant Vice President - Operations Manager
Merrill Lynch May 2006 - Jun 2009
Assistant Vice President - Associate Administrative Manager
Edward Jones Jan 2006 - May 2006
Investment Advisor
HCA Nov 2000 - Dec 2005
Technology Manager
Education:
University of Texas at Tyler 1994 - 1997
BBA, Finance - Concentrations in Information Technology & Portfolio Management
Tyler Junior College 1992 - 1994
Johannesburg Cape Town New York, NY Hong Kong Townsville, Queensland Meadville, PA London, UK
Work:
Ogilvy & Mather - Senior Digital Strategist (2011) WPP Group - Fellow (2008-2011) OgilvyOne Asia Pacific - WPP Fellow (2010-2011) The Jupiter Drawing Room - WPP Fellow (2009-2010) MindShare - WPP Fellow (2008-2009)
Education:
London School of Economics - Communications Regulation and Policy (Research), Allegheny College - English (Creative Writing) and Music (Vocal Performance), James Cook University - Study Abroad
Relationship:
Engaged
Tagline:
Writer/singer/communicator/run... currently living in New York.
Bragging Rights:
I love to write, read, play videogames, run, play tennis, watch movies, talk about anything, travel anywhere and everywhere, eat, and so much more!
Michael Mcclelland
Work:
Car tex trailer (2012-2013)
Education:
Martinsville high school texas
Michael Mcclelland
About:
The CEO Of NeverLessInc
Tagline:
NeverLessIncorporated
Bragging Rights:
SuperProducer
Michael Mcclelland
Education:
Pennsylvania State University - Communication Arts and Sciences