Skills:
Laboratory Skills: Molecular Biology DNA purification, RNA Purification, miRNA Purification, Reverse-Transcriptase PCR, Relative Quantitative PCR, Quantitative Real-Time PCR (SYBR), PCR Mediated miRNA Detection and Quantification, Genotyping, Primer Design, Genomic Cloning via PCR, Restriction Enzyme Digestion, DNA/Plasmid Ligation, Plasmid/siRNA Transfection, Production of Competent Bacterial Cells, Bacteria Transformation, Inducible Protein Expression, Cre-Lox Mediated Gene Excision, rLentiviral Transduction, Exogenous Protein Expression, Genome Sequence Analysis, Protein Purification, FRET-Protein Analysis, Luminescence Activity Assay, Fluorescent Flow Cytometry and Cell Sorting. Tissue Culture Primary Cell Culture, Immortalized Cell Line Culture, Organotypic Tissue Culture, Neuron Cell Culture, Long-Term Tissue, Cell, or Sperm Banking. Assisted Reproductive Technologies Cytoplasmic Microinjection, Perivitelline Space Microinjection, Surgical and Non-Surgical Embryo Collection (Livestock and Rodent), Surgical and Non-Surgical Embryo Transfer (Livestock), Embryo Culture (KSOMaa Evolve, SOF, G1/G2), Oocyte Collection and Maturation, Embryo Cryopreservation, Semen Cryopreservation.