Michael A Watkins

US Patent:

51871076, Feb 16, 1993

Filed:

Jun 27, 1991

Appl. No.:

7/722034

Inventors:

Michael I. Watkins - Hercules CA
Clifford R. Bartlett - Fairfield CA
Edward T. Liang - Hercules CA
John M. Pocekay - Napa CA
Mark A. Staples - Richmond CA

Assignee:

Bio-Rad Laboratories, Inc. - Hercules CA

International Classification:

G01N 33567
G01N 100

US Classification:

436505

Abstract:

Denaturing agents used to pretreat test samples for immunoassays are neutralized and deactivated by the addition of a treatment agent which is a combination of a buffering agent in acid form and an agent for converting the sulfhydryl groups of the denaturing agents to a non-active form such as disulfides or alkylthio groups. The treatment agent serves this function without having sufficient denaturing activity by itself to interfere with subsequent steps of the assay procedure. Also disclosed is a protocol for a vitamin B. sub. 12 assay which involves a sequential rather than competitive binding of the B. sub. 12 with excess intrinsic factor followed by the binding of excess enzyme-labeled B. sub. 12 to any remaining intrinsic and immobilizing such intrinsic factor on a solid phase. Various problems associated with B. sub.

Address:

714 Markwood Dr, Duncanville, TX 75116

Phone:

(972)2985797

VIN:

1N4AL21E97N413961

Make:

NISSAN

Model:

ALTIMA

Year:

2007

Address:

2415 Campfire Crk Dr, Arlington, TX 76018

Phone:

(817)4661984

VIN:

5GZCZ53487S810455

Make:

SATURN

Model:

VUE

Year:

2007