Michael R Yee

US Patent:

7804594, Sep 28, 2010

Filed:

Nov 2, 2007

Appl. No.:

11/934277

Inventors:

Giacomo Vacca - Santa Clara CA, US
Norman R. Goldblatt - Los Altos CA, US
Michael W. Yee - Mount Shasta CA, US

Assignee:

Abbott Laboratories, Inc. - Abbott Park IL

International Classification:

G01N 21/00

US Classification:

356337, 356338

Abstract:

A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

US Patent:

8159670, Apr 17, 2012

Filed:

Oct 31, 2008

Appl. No.:

12/262431

Inventors:

Giacomo Vacca - Santa Clara CA, US
Richard G. Kendall - Miami FL, US
Norman R. Goldblatt - Los Altos CA, US
Michael W. Yee - Mount Shasta CA, US
Mahesh R. Junnarkar - San Jose CA, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 21/00

US Classification:

356337, 356338

Abstract:

A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, and for further reducing the complexity of such a cytometer or analyzer, by utilizing the technique of laser rastering in combination with a lysis-free single-dilution method. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. A lysis-free single-dilution method involves performing all the flow cytometer measurements on a sample using a single aliquot, a single lysis-free reagent solution, a single dilution, and a single pass of said dilution through the measurement apparatus. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flowcell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

US Patent:

8253938, Aug 28, 2012

Filed:

May 26, 2011

Appl. No.:

13/116594

Inventors:

Giacomo Vacca - San Jose CA, US
Norman R. Goldblatt - Mountain View CA, US
Michael W. Yee - Mount Shasta CA, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 21/00

US Classification:

356337, 356338

Abstract:

A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

US Patent:

8400632, Mar 19, 2013

Filed:

Apr 16, 2012

Appl. No.:

13/448216

Inventors:

Giacomo Vacca - Santa Clara CA, US
Richard G. Kendall - Miami FL, US
Norman R. Goldblatt - Los Altos CA, US
Michael W. Yee - Mount Shasta CA, US
Mahesh R. Junnarkar - San Jose CA, US

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 21/00

US Classification:

356337, 356338

Abstract:

A method for increasing the throughput and/or the precision of a flow cytometer, or a hematology analyzer employing a flow cytometer, and for further reducing the complexity of such a cytometer or analyzer. The system and method includes utilizing the technique of laser rastering in combination with a lysis-free single-dilution method.

US Patent:

8045162, Oct 25, 2011

Filed:

Sep 13, 2010

Appl. No.:

12/880523

Inventors:

Giacomo Vacca - San Jose CA, US
Norman R. Goldblatt - Mountain View CA, US
Michael W. Yee - Mount Shasta CA, US

Assignee:

Abbott Laboratories, Inc. - Abbott Park IL

International Classification:

G01N 21/00

US Classification:

356337, 356338

Abstract:

A method for increasing the throughput, or the precision, or both the precision and the throughput, of a flow cytometer, or of a hematology analyzer employing a flow cytometer, by utilizing the technique of laser rastering. Laser rastering involves sweeping a laser beam across a flowing sample stream in a hematology analyzer. An apparatus suitable for carrying out the method of this invention comprises an optical module comprising a source of light, a scanning device, a lens or system of lenses, a flow cell, detectors, and filters; and an electronic module comprising preamplifiers, analog signal conditioning elements, analog-to-digital converters, field-programmable gate arrays, digital signal processing elements, and data storage elements.

US Patent:

55590373, Sep 24, 1996

Filed:

Dec 15, 1994

Appl. No.:

8/356932

Inventors:

Young R. Kim - Sunnyvale CA
Michael W. Yee - Sunnyvale CA
Suresh N. Mehta - Pleasanton CA
Josefino C. Sagala - San Jose CA
Johanna Kantor - Palo Alto CA

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 3348

US Classification:

436 63

Abstract:

A method and a device for the simultaneous and quantitative, flow cytometric analysis of nucleated red blood cells (NRBC) and white blood cells (WBC) in a whole blood sample. The method includes the lysis of red blood cells (RBC) and NRBC cytoplasm from an aliquot of a whole blood sample to eliminate RBC and to expose the NRBC to a vital nuclear stain and the inhibition of the permeation of the nuclear stain into the WBC, subjecting the stained aliquot to flow cytometric light measurements, obtaining at least one signal for parameters including scattered light at a first and a second range of scatter angles and fluorescence (FL), qualifying the obtained signals by using the combination logic wherein a qualified signal must be greater than the second scatter signal threshold, while at the same time it must be greater than either the first scatter signal threshold or the FL threshold {[(first scatter angle signal OR FL signals) AND second scatter angle signal]}, constructing a three-dimensional plot of qualified intensity signals of fluorescence and scattered light from the detected signals, and differentiating the NRBC and WBC from the constructed three-dimensional plot and determining the number of cells of each.

US Patent:

58799001, Mar 9, 1999

Filed:

May 5, 1997

Appl. No.:

8/851526

Inventors:

Young Ran Kim - Sunnyvale CA
Michael W. Yee - Sunnyvale CA
Suresh N. Mehta - Pleasanton CA
Josefino C. Sagala - San Jose CA

Assignee:

Abbott Laboratories - Abbott Park IL

International Classification:

G01N 33569

US Classification:

435724

Abstract:

A method for the simultaneous and quantitative, flow cytometric analysis of nucleated red blood cells (NRBC), white blood cells (WBC), damaged white blood cells and a white blood cell subclass differential (WBC/Diff) is provided. The method includes mixing an aliquot of a whole blood sample with a reagent system comprising a red blood cell (RBC) lysing component which lyses RBCs and NRBCs while minimizing damage to WBC cellular membranes and a membrane-impermeant nucleic acid stain which stains exposed NRBC nuclei and damaged WBCs, subjecting the stained aliquot to flow cytometric light measurements, obtaining at least one signal for the parameters of fluorescence (FL) and scattered light at a first and a second range of scatter angles, qualifying the obtained signals using AND/OR logic wherein to be qualified, a signal must be greater than the second scatter signal threshold AND also greater than either the first scatter signal threshold OR the FL threshold, constructing a three-dimensional plot of qualified intensity signals of fluorescence and scattered light from the detected signals, and differentiating the NRBC, WBC, damaged WBC and WBC/Diff from the constructed three-dimensional plot and determining the number of cells of each.