Michele A Cramer

US Patent:

7491545, Feb 17, 2009

Filed:

Sep 30, 2003

Appl. No.:

10/673409

Inventors:

Richard Thompson - Baltimore MD, US
Michele Cramer - Baltimore MD, US
Carol Ann Fierke - Ann Harbor MI, US
Hui Hui Zeng - Ellicott City MD, US
Rebecca Bozym - Moon Township PA, US

International Classification:

G01N 33/20

US Classification:

436 81, 435 15, 435 18, 436 73, 436 74, 436166, 436172

Abstract:

A highly selective and sensitive of a carbonic anhydrase-based method for measurement of zinc ion by an excitation ratiometric format based on resonance energy transfer: i. e. , where the zinc ion level is transduced as the ratio of fluorescence intensities excited at two different excitation wavelengths, is provided. The method can be used very well in a fluorescence microscopy format. A detection limit of about 10 pM in zinc buffered systems, a ten to one thousand-fold improvement on the Fura indicators (which respond to Ca and Mg as well), and a one hundred thousand-fold improvement on the recently described FuraZin-1 is achieved.