Michelle R Butler

US Patent:

20080032343, Feb 7, 2008

Filed:

Dec 19, 2006

Appl. No.:

11/613012

Inventors:

Michelle Butler - Belmont CA, US
Jeffrey Cleland - San Carlos CA, US
David Kahn - North Potomac MD, US
Shelly Pizarro - San Carlos CA, US
Charles Schmelzer - Burlingame CA, US
Marjorie Winkler - Burlingame CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12P 21/00

US Classification:

435071200

Abstract:

A process for recovering and purifying refolded heparin binding proteins produced in heterologous host cells includes the step of incubation of the solubilized protein with a polyanionic species such as dextran sulfate.

US Patent:

20100261888, Oct 14, 2010

Filed:

Jun 17, 2010

Appl. No.:

12/817382

Inventors:

Michelle D. Butler - San Francisco CA, US
Jeffrey L. Cleland - San Carlos CA, US
David W. Kahn - North Potomac MD, US
Shelly Pizarro - San Carlos CA, US
Charles H. Schmelzer - Burlingame CA, US
Marjorie E. Winkler - Burlingame CA, US

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C07K 1/14

US Classification:

530399, 530412

Abstract:

A process for recovering and purifying refolded heparin binding proteins produced in heterologous host cells includes the step of incubation of the solubilized protein with a polyanionic species such as dextran sulfate.

US Patent:

63132850, Nov 6, 2001

Filed:

Jul 21, 2000

Appl. No.:

9/620849

Inventors:

Michelle D. Butler - San Francisco CA
Darien L. Cohen - Oakland CA
David Kahn - Foster City CA
Marjorie E. Winkler - Burlingame CA

Assignee:

Genentech, Inc. - South San Francisco CA

International Classification:

C12N 1510

US Classification:

536 254

Abstract:

A process is described for purifying plasmid DNA from prokaryotic cells comprised thereof. This process comprises the steps of: (a) digesting the cells; (b) incubating the cells for about 4 to 24 hours to effect lysis and solubilization thereof, without effecting enzymatic digestion of RNA; (c) removing lysate contaminants from the cells to provide a plasmid DNA solution; (d) filtering the solution through a tangential flow filtration device to obtain a retentate containing the plasmid DNA; and (e) collecting the retentate.