Min Li

US Patent:

8170805, May 1, 2012

Filed:

Feb 6, 2009

Appl. No.:

12/367045

Inventors:

Venkata Krishna Kishore - Bloomington IL, US
Zhigang Guo - Champaign IL, US
Min Li - Champaign IL, US
Daolong Wang - Normal IL, US
Libardo Andres Gutierrez Rojas - Northfield MN, US
Joseph Byrum - West Des Monies IA, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

C12Q 1/68
G01N 33/48
G06G 7/58

US Classification:

702 19, 435 6, 702 20, 703 2, 703 11

Abstract:

Provided herein are methods for evaluating associations between candidate genes and a trait of interest in a population. The methods include a combination of genome-wide association analysis and one or more of nested association mapping (NAM), expression QTL analysis (eQTL), and allele epistastic analysis (AEA). Markers are selected or prioritized if they are shown to be positively-correlated with a trait of interest using GWA and a combination of one or both of NAM and eQTL. Also provided are models for evaluating the association between a candidate marker and a trait in a nested population of organisms. These methods include single marker regression and multiple marker regression models. Markers identified using the methods of the invention can be used in marker assisted breeding and selection, as genetic markers for constructing linkage maps, for gene discovery, for identifying genes contributing to a trait of interest, and for generating transgenic organisms having a desired trait.

US Patent:

20100269216, Oct 21, 2010

Filed:

Apr 16, 2009

Appl. No.:

12/425122

Inventors:

Zhigang Guo - Champaign IL, US
Venkata Krishna Kishore - Bloomington IL, US
Suresh Babu Kadaru - Northfield MN, US
Min Li - Champaign IL, US
Todd Lee Warner - Eagan MN, US
Homer Gene Caton - Madrid IA, US

Assignee:

Syngenta Participations AG - Basel

International Classification:

A01H 1/06
C12Q 1/68
A01H 5/00

US Classification:

800278, 435 6, 800295

Abstract:

Provided herein are methods for mapping quantitative trait loci in a connected population of organisms. The invention includes evaluating associations between markers and a trait of interest using network population mapping (NPM). The methods include assembling a network of individual members for association mapping, wherein the members are connected at the allelic level. Members of the network are grouped according to a shared haplotype at one or more marker loci, and the network can be used to identify or validate QTL within the chromosomal region surrounding or flanked by the marker loci. The methods further include a means for estimating and ranking the effects of multiple alleles across the mapping population. Further provided is a novel simple interval mapping model as well as a novel composite interval mapping model for evaluating allele-specific associations across a connected mapping population.