Nada A Bsat

US Patent:

8541224, Sep 24, 2013

Filed:

Jan 2, 2013

Appl. No.:

13/732870

Inventors:

Kerry Roche Lentine - Tyngsboro MA, US
Nada Bsat - Bedford MA, US

Assignee:

EMD Millipore Corporation - Billerica MA

International Classification:

C12N 1/20
C12Q 1/02
C12Q 1/22

US Classification:

4352536, 435 29, 435 31, 4352521

Abstract:

A method for retention testing sterilizing grade filters comprises: a) providing a stock of ; b) growing up the stock of for about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration of in the filtrate. Serum-free growth media for cultivating or storing are also described.

US Patent:

20100221771, Sep 2, 2010

Filed:

Jul 30, 2008

Appl. No.:

12/675318

Inventors:

Shawn Bates - Boston MA, US
Kerry Roche Lentine - Tyngsboro MA, US
Nada Bsat - Bedford MA, US

International Classification:

C12Q 1/04
C12N 1/20

US Classification:

435 34, 4352536

Abstract:

A method for retention testing sterilizing grade filters comprises: a) providing a stock of ; b) growing up the stock of for about 24 hours or less in a single serum-free growth medium that supports cell growth to a high titer and yields a cellular morphology where the cells are small, deaggregated and spherical, thereby producing a bacterial culture; c) challenging a test filter by filtering the bacterial culture through the test filter at a known challenge level, thereby producing a filtrate downstream of the test filter; and d) detecting concentration of in the filtrate. Serum-free growth media for cultivating or storing are also described.