Nancy Peck Hanson

US Patent:

7476520, Jan 13, 2009

Filed:

Mar 21, 2005

Appl. No.:

11/084973

Inventors:

Nancy D. Hanson - Gretna NE, US
Christine C. Sanders - Englewood FL, US
Anton F. Ehrhardt - Omaha NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12P 19/34
C12Q 1/68
C07H 21/02
C07H 21/04
C07H 21/00

US Classification:

435 912, 435 6, 435 911, 536 231, 536 243, 536 2433, 536 253

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain beta-lactamases. The primers can be employed in methods to identify nucleic acid characteristic of family-specific beta-lactamase enzymes in samples, and particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

7521547, Apr 21, 2009

Filed:

Dec 13, 2005

Appl. No.:

11/302037

Inventors:

Nancy Hanson - Gretna NE, US
Francisco-Javier Perez-Perez - Victoria, ES

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

536 231, 435 6, 435975

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain AmpC beta-lactamases. The primers can be employed in methods to detect the presence or absence of an AmpC beta-lactamase gene in samples, and to identify nucleic acid characteristic of AmpC beta-lactamase genes in samples, particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

20030219749, Nov 27, 2003

Filed:

May 17, 2002

Appl. No.:

10/147601

Inventors:

Nancy Hanson - Gretna NE, US
Francisco-Javier Perez - Vitoria, ES

Assignee:

Creighton University

International Classification:

C12Q001/68
C12P019/34

US Classification:

435/006000, 435/091200

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain AmpC beta-lactamases. The primers can be employed in methods to detect the presence or absence of an AmpC beta-lactamase gene in samples, and to identify nucleic acid characteristic of AmpC beta-lactamase genes in samples, particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

20040002080, Jan 1, 2004

Filed:

Dec 13, 2002

Appl. No.:

10/318970

Inventors:

Nancy Hanson - Gretna NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435/006000, 435/091200, 536/024300

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain beta-lactamases. The primers can be employed in methods to identify nucleic acid characteristic of family-specific beta-lactamase enzymes in samples, and particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

20070077562, Apr 5, 2007

Filed:

Jul 21, 2004

Appl. No.:

10/565358

Inventors:

Ashfaque Hossain - Omaha NE, US
Nancy Hanson - Gretna NE, US
Mark Reisbig - Omaha NE, US

International Classification:

C12Q 1/68
C07H 21/02

US Classification:

435006000, 536025400

Abstract:

A method of isolating RNA from a biological specimen is provided, whereby a biological specimen is contacted with an admixture of (i) a mono-phasic solution of phenol and guanidine isothiocyanate and (ii) a lysis buffer under conditions and for a time appropriate to form a homogenate. Next, the homogenate is admixed with a water-immiscible organic solvent under conditions and for a time appropriate to form an aqueous phase and an organic phase. The aqueous phase is then contacted with a C-Clower alcohol under conditions and for a time to form a precipitated RNA. The precipitated RNA is then recovered by centrifugation and decanting of the aqueous phase. The method can also be used to isolate total RNA. In an alternative embodiment, the biological sample is contacted with (i) a lysis buffer, and (ii) a mono-phasic solution of phenol and guanidine isothiocyanate under conditions and for a time appropriate to form a homogenate. The remaining steps of this embodiment are the same as above.

US Patent:

20070248954, Oct 25, 2007

Filed:

Sep 10, 2004

Appl. No.:

10/571027

Inventors:

Nancy Hanson - Gretna NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435006000, 536024330

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain β-lactamase genes. The primers can be employed in methods to identify nucleic acid characteristic of family-specific (and even group-specific) β-lactamase enzymes in samples, and particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

20090317807, Dec 24, 2009

Filed:

May 25, 2007

Appl. No.:

12/225841

Inventors:

Nancy D. Hanson - Gretna NE, US

Assignee:

Creighton University - Omaha NE

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 6, 536 2433

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain beta-lactamases. The primers can be employed in methods to identify nucleic acid characteristic of family-specific beta-lactamase enzymes in samples, and particularly, in clinical isolates of Gram-negative bacteria.

US Patent:

20110311976, Dec 22, 2011

Filed:

Nov 20, 2008

Appl. No.:

12/274770

Inventors:

NANCY D. HANSON - GRETNA NE, US
CHRISTINE C. SANDERS - ENGLEWOOD FL, US
ANTON F. EHRHARDT - OMAHA NE, US

Assignee:

CREIGHTON UNIVERSITY - OMAHA NE

International Classification:

C12Q 1/68
C07H 21/00

US Classification:

435 612, 536 2433

Abstract:

Oliognucleotide primers are provided that are specific for nucleic acid characteristic of certain beta-lactamases. The primers can be employed in methods to identify nucleic acid characteristic of family-specific beta-lactamase enzymes in samples, and particularly, in clinical isolates of Gram-negative bacteria.