Nancy T Harding

US Patent:

7868167, Jan 11, 2011

Filed:

Nov 1, 2005

Appl. No.:

11/264268

Inventors:

Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
Russell Coleman - San Diego CA, US
Steven Matzke - San Diego CA, US

Assignee:

CP Kelco U.S., Inc. - Atlanta GA

International Classification:

C08B 37/00
C12P 19/04

US Classification:

536114, 536123, 536124, 514782, 435101

Abstract:

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention.

US Patent:

7887866, Feb 15, 2011

Filed:

Mar 2, 2001

Appl. No.:

09/798642

Inventors:

Stan Bower - La Jolla CA, US
Ellen Burke - San Diego CA, US
Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
J. Carrie Schneider - San Diego CA, US
Dagmar Meissner - San Diego CA, US
Neil A. Morrison - San Diego CA, US
Ralph Bezanson - Alpine CA, US

Assignee:

CP Kelco U.S., Inc. - Atlanta GA

International Classification:

A23L 1/054

US Classification:

426573, 426271

Abstract:

The invention relates to mutant strains of the genus which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30 C. to about 70 C. , and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

US Patent:

7888333, Feb 15, 2011

Filed:

Feb 3, 2006

Appl. No.:

11/347341

Inventors:

Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
Russell J. Coleman - San Diego CA, US

Assignee:

CP Kelco U.S., Inc. - Atlanta GA

International Classification:

A61K 31/715
C08B 37/00

US Classification:

514 54, 536123

Abstract:

The present invention provides improved diutan polysaccharides produced by mutant strains containing at least one genetic modification that favors slime-forming diutan production that exhibits increased viscosity. Methods of making the mutant strains and the culture broth containing such mutant strains are also provided.

US Patent:

8198064, Jun 12, 2012

Filed:

Dec 2, 2005

Appl. No.:

11/292366

Inventors:

Stan Bower - La Jolla CA, US
Ellen Burke - San Diego CA, US
Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
J. Carrie Schneider - San Diego CA, US
Dagmar Meissner - San Diego CA, US
Neil A. Morrison - San Diego CA, US
Ralph Bezanson - Alpine CA, US

Assignee:

CP Kelco U.S., Inc. - Atlanta GA

International Classification:

C12N 1/12
C12N 15/00
C12N 9/10
C07H 21/02
C07H 21/04

US Classification:

4352521, 435440, 435193, 536 231, 536 232

Abstract:

The invention relates to mutant strains of the genus which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30 C. to about 70 C. , and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.

US Patent:

8278438, Oct 2, 2012

Filed:

Sep 28, 2010

Appl. No.:

12/892561

Inventors:

Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
Russell Coleman - San Diego CA, US
Steven Matzke - San Diego CA, US

Assignee:

CP Kelco U.S., Inc. - Atlanta GA

International Classification:

C08B 37/00
C12P 19/04

US Classification:

536114, 536123, 536124, 514782, 435101

Abstract:

The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of sp. ATCC 53159 that harbors a multicopy broad-host-range plasmid into which genes for biosynthesis of diutan polysaccharide have been cloned. The plasmid provides the capability within the host strain to produce multiple copies of genes for such polysaccharide synthesis. In such a manner, a method of not just increased production of the target diutan polysaccharide, but also production of a diutan polysaccharide of improved physical properties (of the aforementioned higher viscosity) thereof is provided. Such a diutan polysaccharide has proven particularly useful as a possible viscosifier in oilfield applications and within cement materials. The inventive methods of production of such an improved diutan polysaccharide, as well as the novel cloned genes required to produce the improved diutan within such a method, are also encompassed within this invention.

US Patent:

7361754, Apr 22, 2008

Filed:

Jun 20, 2005

Appl. No.:

11/156953

Inventors:

Joseph M. Cleary - Carlsbad CA, US
Russell J. Coleman - San Diego CA, US
Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US

Assignee:

CP Kelco US, Inc. - Atlanta GA

International Classification:

C07G 17/00
C12N 1/20
C12N 15/87
C09K 3/00
A23C 9/154
A23C 9/12

US Classification:

536114, 426 34, 426580, 426 36, 4352523, 435463, 516105

Abstract:

Mutational inactivation of proteins involved in para-cresol production in certain milk products results in improved taste and odor. The undesirable para-cresol forms over time as a result of enzymes produced by the bacterium that produces gellan gum. Since the gellan is typically used in a relatively unpurified form, the enzymes are added to the milk along with the gellan. Inactivation of the enzymes is a genetic means of eliminating the enzymes without requiring any additional purification or processing.

US Patent:

20030100078, May 29, 2003

Filed:

Jul 3, 2002

Appl. No.:

10/188092

Inventors:

Nancy Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
John McQuown - San Diego CA, US

International Classification:

C12P019/04
C08B037/00
C12N001/21

US Classification:

435/101000, 435/252300, 536/123000

Abstract:

This invention provides a mutant Sphingomonas microorganism which produces a polysaccharide polymer comprising repeating tetramer units having a D-glucose:D-glucuronic acid:L-rhamnose ratio of about 2:1:1, wherein the D-glucose moieties are linked in a -[1,4] configuration to the D-glucuronic acid moiety. One of the D-glucose moieties is linked to the L-rhamnose moiety in an -[1,3] configuration, the other glucose moiety is linked to the L-rhamnose moiety in a -[1,4] configuration, wherein the polysaccharide polymer is substantially non-acetylated. This invention also provides the substantially non-acetylated polymer, as well as process for obtaining the substantially non-acetylated polymer.

US Patent:

20080268527, Oct 30, 2008

Filed:

Dec 2, 2005

Appl. No.:

11/292356

Inventors:

Stan Bower - La Jolla CA, US
Ellen Burke - San Diego CA, US
Nancy E. Harding - San Diego CA, US
Yamini N. Patel - San Diego CA, US
J. Carrie Schneider - San Diego CA, US
Dagmar Meissner - San Diego CA, US
Neil A. Morrison - San Diego CA, US
Ralph Bezanson - Alpine CA, US

Assignee:

C.P. Kelco U.S., Inc. - Wilmington DE

International Classification:

C08B 37/00

US Classification:

435274

Abstract:

The invention relates to mutant strains of the genus which have a mutation in at least one gene encoding a protein involved in polyhydroxybutyrate (“PHB”) synthesis that allows the mutant strains to produce PHB-deficient sphingans. The invention is also directed to a process for preparing a clarified sphingan solution comprising heating aqueous sphingan solution, in particular PHB-deficient sphingan solution, to a clarification temperature of about 30 C. to about 70 C., and treating the solution with a clarification agent and enzymes. In addition, the invention is directed to a food or industrial product comprising a PHB-deficient and/or clarified sphingan. One particular embodiment of the invention is directed to a clarified, PHB-deficient high-acyl gellan and the processes of making thereof.