Natalia Orlova

US Patent:

20200309701, Oct 1, 2020

Filed:

Jun 12, 2020

Appl. No.:

16/900279

Inventors:

Dmitri TSYBOULSKI - Seattle WA, US
Natalia ORLOVA - Seattle WA, US
Jerome Anthony LECOQ - Seattle WA, US
Peter SAGGAU - Seattle WA, US

Assignee:

Allen Institute - Seattle WA

International Classification:

G01N 21/64
G02B 21/00
G02B 21/36

Abstract:

A system includes a light source to generate an optical signal having a set of pulses at a first repetition rate. The system also includes a multiplexer circuit to generate a multiplexed optical signal from the optical signal n sets of pulses at a second repetition rate, where the n sets of pulses have different polarization states and are at the first repetition rate. The system also includes a focusing unit to split the multiplexed optical signal into n excitation signals to excite a sample. The system also includes an objective to receive the n excitation signals and to illuminate the sample. The objective and the focusing unit collectively focus each excitation signal of the n excitation signals on a different focal plane of the sample to generate a response signal. The system also includes a demultiplexer circuit to generate n emission signals based on the response signal.