Nathan G Harris

US Patent:

8450118, May 28, 2013

Filed:

Apr 28, 2010

Appl. No.:

12/799689

Inventors:

Scot Weinberger - Montara CA, US
Stephen Dotson - Montara CA, US
Nathan Harris - Morgan Hill CA, US

Assignee:

Molecular Sensing, Inc. - Montara CA

International Classification:

G01N 33/00
G01N 21/75

US Classification:

436 86, 436164, 436165, 436166, 436 63, 435 71

Abstract:

Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.