Neil G Balgobin

US Patent:

20030032146, Feb 13, 2003

Filed:

Jul 10, 2001

Appl. No.:

09/901996

Inventors:

Francis Dwulet - Greenwood IN, US
Neil Balgobin - Carmel IN, US
Robert McCarthy - Carmel IN, US

International Classification:

C12P021/04
C12P021/02
C07K001/22

US Classification:

435/069700, 530/413000, 435/068100, 530/344000

Abstract:

A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a specific sequence of amino acids that has the desired affinity for a non-antibody capture protein. This peptide maybe linked to either the amino or carboxyl terminal of the functional protein. The identification peptide may or may not contain a sequence of amino acids that can be fragmented by sequence specific proteases or chemical agents to yield the native protein. The hybrid polypeptide produced by either cell or cell-free based expression systems is now suitable for further processing. This fusion protein can be purified by affinity chromatographic techniques using an immobilized non-antibody capture protein that has the desired affinity for the identification peptide. Alternately the capture protein may contain a label that will allow for the tracking of the functional protein in the system being studied.

US Patent:

20040110255, Jun 10, 2004

Filed:

Sep 12, 2003

Appl. No.:

10/661207

Inventors:

Francis Dwulet - Greenwood IN, US
Neil Balgobin - Carmel IN, US
Robert McCarthy - Carmel IN, US

International Classification:

C12P021/04
C12N009/00

US Classification:

435/069700, 435/183000, 435/320100, 435/325000

Abstract:

A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a specific sequence of amino acids that has the desired affinity for a non-antibody capture protein. This peptide maybe linked to either the amino or carboxyl terminal of the functional protein. The identification peptide may or may not contain a sequence of amino acids that can be fragmented by sequence specific proteases or chemical agents to yield the native protein. The hybrid polypeptide produced by either cell or cell-free based expression systems is now suitable for further processing. This fusion protein can be purified by affinity chromatographic techniques using an immobilized non-antibody capture protein that has the desired affinity for the identification peptide. Alternately the capture protein may contain a label that will allow for the tracking of the functional protein in the system being studied.

US Patent:

20050214900, Sep 29, 2005

Filed:

Feb 23, 2005

Appl. No.:

11/063355

Inventors:

Francis Dwulet - Greenwood IN, US
Neil Balgobin - Carmel IN, US
Robert McCarthy - Carmel IN, US

International Classification:

C12P021/06

US Classification:

435068100

Abstract:

A hybrid polypeptide composed of an identification peptide and a desired functional protein are produced by recombinant DNA techniques. A DNA expression vector is constructed that includes segments of DNA coding for the identification peptide and the desired functional protein. The identification peptide consists of a specific sequence of amino acids that has the desired affinity for a non-antibody capture protein. This peptide maybe linked to either the amino or carboxyl terminal of the functional protein. The identification peptide may or may not contain a sequence of amino acids that can be fragmented by sequence specific proteases or chemical agents to yield the native protein. The hybrid polypeptide produced by either cell or cell-free based expression systems is now suitable for further processing. This fusion protein can be purified by affinity chromatographic techniques using an immobilized non-antibody capture protein that has the desired affinity for the identification peptide. Alternately the capture protein may contain a label that will allow for the tracking of the functional protein in the system being studied.

US Patent:

54459338, Aug 29, 1995

Filed:

Sep 15, 1992

Appl. No.:

7/945156

Inventors:

James S. Eadie - Indianapolis IN
Neil G. Balgobin - Carmel IN
Harvey Buck - Weilheim, DE

Assignee:

Boehringer Mannheim Corporation - Indianapolis IN

International Classification:

C12Q 168

US Classification:

435 6

Abstract:

The invention relates to an improvement in the strand displacement assay. Probes that are at least 20 nucleotide bases in length, but are no more than 40 bases in length, are used. These short probes permit detection and discrimination down to the order of a single base.