Ning Liu

US Patent:

20100125128, May 20, 2010

Filed:

Nov 9, 2009

Appl. No.:

12/615080

Inventors:

Ning Liu - Fremont CA, US
Katrina Academia - Hercules CA, US
Aran Paulus - San Jose CA, US
Tim Wehr - Albany CA, US
Steve Freeby - Vacaville CA, US

Assignee:

BIO-RAD LABORATORIES, INC. - Hercules CA

International Classification:

C07K 1/14

US Classification:

530344

Abstract:

Phosphorylated peptides are extracted from digests of biological liquids and other peptide mixtures by fractionation on ceramic hydroxyapatite. The ceramic hydroxyapatite is readily usable in a centrifuge, allowing for rapid fractionations of a large number of small volume samples, and accordingly high throughput.

US Patent:

20130288388, Oct 31, 2013

Filed:

Apr 25, 2013

Appl. No.:

13/870710

Inventors:

Ning Liu - Fremont CA, US
Kevin McDonald - Novato CA, US
Aran Paulus - San Jose CA, US
Anton Posch - Grafting, DE

Assignee:

Bio-Rad Laboratories, Inc., LSG - LSD Division - Hercules CA

International Classification:

G01N 33/53

US Classification:

436501

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20190178877, Jun 13, 2019

Filed:

Oct 12, 2018

Appl. No.:

16/158853

Inventors:

- Herculer CA, US
Ning LIU - Fremont CA, US
Kevin McDONALD - Novato CA, US
Aran PAULUS - San Jose CA, US
Anton POSCH - Grafing, DE

International Classification:

G01N 33/53
G16B 99/00
G06T 11/60
G01N 33/68
G06T 7/00

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20170030922, Feb 2, 2017

Filed:

Oct 13, 2016

Appl. No.:

15/293154

Inventors:

- Hercules CA, US
Ning Liu - Fremont CA, US
Kevin McDonald - Novato CA, US
Aran Paulus - San Jose CA, US
Anton Posch - Grafing, DE

International Classification:

G01N 33/68
G06T 7/00
G06T 11/60
G06F 19/10

Abstract:

Disclosed herein are methods of protein quantification and normalization using haloalkylated tryptophan fluorescence. Complex protein samples, i.e., samples that each contain 1,000 or more distinct proteins, from diverse sources that do not have common protein profiles are treated with a halo-substituted organic compound (i.e. haloalkane) that reacts with tryptophan residues to form fluorescent products. Irradiation of the samples with ultraviolet light and the detection and quantification of the resultant fluorescent emissions from all proteins in each sample are then used to obtain comparative values for total protein content among the various samples. The values thus obtained are found to be valid indications of comparative total protein content, despite the fact that the tryptophan levels vary widely among the various proteins in any single sample and the samples, due to the diversity of their origins, tend to differ among themselves in the identities and relative amounts of the proteins that they contain. Protein samples are also normalized to correct for differences in sample dilution, sample loading, and protein transfer inconsistencies, by using stain-free detection of total protein in each of the samples, or detection of subsamples within each sample.

US Patent:

20150044144, Feb 12, 2015

Filed:

Mar 6, 2013

Appl. No.:

14/376434

Inventors:

- Pleasanton CA, US
Ning Liu - Fremont CA, US

Assignee:

RAYMAT MATERIALS, INC. - Pleasanton CA

International Classification:

A01N 25/00
A61K 33/20
A61K 47/02
A01N 59/00

US Classification:

424 103

Abstract:

The present invention relates to a disinfectant solution, comprising an oxidative and reductive potential (ORP) solution and a colorant. A disinfectant article, a method of forming the disinfectant solution and use of the disinfectant solution are also provided.