- Santa Clara CA, US Paige Anderson - Mountain View CA, US Javelin Chi - Sunnyvale CA, US Henrik Johansson - Stockholm, SE
International Classification:
C12N 15/10 C12Q 1/68
Abstract:
The present invention relates to nucleic acid samples for massively parallel sequencing. More particularly, the present invention relates to assay methods, compositions and kits for detecting contamination of nucleic acid identifiers such as sample barcodes.
Composition And Method For Improving Signal-To-Noise In Hybridization To Oligonucleotide Arrays
- Santa Clara CA, US Paige Anderson - Mountain View CA, US
International Classification:
C12Q 1/68
Abstract:
Provided herein, among other things, are compositions for improving signal-to-noise in hybridization to oligonucleotide arrays. In some embodiments, the composition may comprise a population of indexing oligonucleotides of formula X-Y, where the sequences of region X hybridize to an array and the sequences of region Y hybridize to distinct sites within a genomic region, transcript, set of transcripts, or complement of the same. Use of these oligonucleotides increases signal, reduces background and smoothens array hybridization analyses. Methods and kits that employ the compositions are also provided.
- Loveland CO, US Nicholas M. Sampas - San Jose CA, US Joel Myerson - Berkeley CA, US Paige Anderson - Belmont CA, US Bo Curry - Redwood City CA, US
International Classification:
C12N 15/10 B01J 19/00
Abstract:
Provided herein, among other things, is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence can be assembled to produce a synthon; and (b) hybridizing the oligonucleotide mixture to an array, thereby spatially-separating the different sets of oligonucleotides from one another. Other embodiments are also provided.
Tomahawk Elementary School Lynchburg VA 1974-1976, Leesville Road Elementary School Lynchburg VA 1976-1978, Delmae Heights Elementary School Florence SC 1978-1979, Moore Intermediate School Florence SC 1980-1982
Community:
Gayle Kirby
Biography:
Life
I am a stay at home, homeschooling mom of 6 children; 3 daughters ages 16, 14, ...