Pankaj Qasba - Columbia MD, US Mark A. Thiede - Forest Hill MD, US
Assignee:
Osiris Therapeutics - Baltimore MD
International Classification:
A01N 63/00 A01N 65/00 C12N 5/00
US Classification:
424 9321, 435325, 424 931
Abstract:
The present invention is directed to hematopoietic progenitor cells isolated from a tissue specimen, such as marrow cells or peripheral blood, and to the method of co-culturing isolated hematopoietic progenitor cells with human mesenchymal stem cells to induce megakaryocyte differentiation and platelet production. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate blood disorders.
Production Of Megakaryocytes By The Use Of Human Mesenchymal Stem Cells
Pankaj Qasba - Columbia MD Mark A. Thiede - Forest Hill MD
Assignee:
Osiris Therapeutics, Inc. - Baltimore MD
International Classification:
C12N 500 C12N 508 C12N 506 C12N 510 A01N 6300
US Classification:
435373
Abstract:
The present invention is directed to hematopoietic progenitor cells isolated from a tissue specimen, such as marrow cells or peripheral blood, and to the method of co-culturing isolated hematopoietic progenitor cells with human mesenchymal stem cells to induce megakaryocyte differentiation and platelet production. In addition, hematopoietic stem cells can be genetically engineered to carry genes of interest particularly for the expression of physiologically active proteins. In the presence of mesenchymal stem cells, the transduced cells carry the new genetic material and express gene products that can be used to modulate blood disorders.