Patricia A Hand

US Patent:

46122826, Sep 16, 1986

Filed:

Mar 1, 1985

Appl. No.:

6/707400

Inventors:

Jeffrey Schlom - Vienna VA
David Colcher - Vienna VA
Marianna Nuti - Bethesda MD
Patricia H. Hand - Washington DC
Faye Austin - Annandale VA

Assignee:

The United States of America as represented by the Secretary of the
Department of Health and Human Services - Washington DC

International Classification:

C12P 2100
C12N 1500
C12N 500
G01N 3354

US Classification:

435 68

Abstract:

Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.

US Patent:

45229180, Jun 11, 1985

Filed:

Dec 15, 1981

Appl. No.:

6/330959

Inventors:

Jeffery Schlom - Vienna VA
David Colcher - Vienna VA
Marianna Nuti - Bethesda MD
Patricia H. Hand - Washington DC
Faye Austin - Annandale VA

International Classification:

C12P 2100
C12N 500
C12N 1500
G01N 3354
A61K 4900
A61K 3900
C12R 191

US Classification:

435 68

Abstract:

Monoclonal antibodies demonstrating a reactivity with human breast cancer are produced. The hybridoma cultures secreting immunoglobins are produced by hydridoma technology. Splenic lymphocytes of mice, immunized with membrane-enriched fractions of metastatic human mammary carcinoma tissue are fused with the NS-1 non-immunoglobulin-secreting murine myeloma cell line. Screening of immunoglobulin reactivities and double cloning of cultures yielded 11 monoclonal antibodies that demonstrated activities with the surface of human mammary tumor cells and not with the surface of apparently normal human tissues. These monoclonal antibodies aid in the diagnosis, prognosis and treatment of human breast cancer.