Patricia R Sanders

US Patent:

D551391, Sep 18, 2007

Filed:

Feb 27, 2004

Appl. No.:

29/200388

Inventors:

Patricia J. Sanders - Dolton IL, US

International Classification:

2803

US Classification:

D28 63, D4114

US Patent:

56890524, Nov 18, 1997

Filed:

Sep 19, 1995

Appl. No.:

8/530492

Inventors:

Sherri Marie Brown - Chesterfield MO
Duff Allen Dean - St. Louis MO
Michael Ernest Fromm - Chesterfield MO
Patricia Rigden Sanders - Chesterfield MO

Assignee:

Monsanto Company - St. Louis MO

International Classification:

A01H 500
C12N 1511
C12N 1582

US Classification:

800205

Abstract:

A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided. Also disclosed are novel structural genes which encode insecticidal proteins of B. t. k. and monocotyledonous (e. g.

US Patent:

61807745, Jan 30, 2001

Filed:

Aug 5, 1997

Appl. No.:

8/906517

Inventors:

Sherri Marie Brown - Chesterfield MO
Duff Allen Dean - St. Louis MO
Michael Ernest Fromm - Chesterfield MO
Patricia Rigden Sanders - Chesterfield MO

Assignee:

Monsato Company - St. Louis MO

International Classification:

C07H 2102

US Classification:

536 2371

Abstract:

A method for modifying a foreign nucleotide sequence for enhanced accumulation of its protein product in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by reducing the frequency of the rare and semi-rare monocotyledonous codons in the foreign gene and replacing them with more preferred monocotyledonous codons is disclosed. In addition, a method for enhancing the accumulation of a polypeptide encoded by a nucleotide sequence in a monocotyledonous plant and/or increasing the frequency of obtaining transgenic monocotyledonous plants which accumulate useful amounts of a transgenic protein by analyzing the coding sequence in successive six nucleotide fragments and altering the sequence based on the frequency of appearance of the six-mers as to the frequency of appearance of the rarest 284, 484, and 664 six-mers in monocotyledonous plants is provided. Also disclosed are novel structural genes which encode insecticidal proteins of B. t. k. and monocotyledonous (e. g.