Patrick Gilles - Carlsbad CA, US Patrick Dillon - Carlsbad CA, US David Wu - La Jolla CA, US Charles Foster - Silver Spring MD, US Stephen Chanock - Bethesda MD, US
International Classification:
C12Q001/68
US Classification:
435/006000
Abstract:
A rapid assay for single nucleotide polymorphism (SNP) detection that utilizes electronic circuitry on silicon microchips is disclosed. The method provides accurate discrimination of amplified DNA samples following electronic assisted transport, concentration, and attachment of DNA to selected electrodes (test sites). The test sites make up organized arrays of samples that are distinguished by using internal controls of dual labeled reporters comprising wild-type and mismatched sequences to validate the SNP genotype. This method has been used to discriminate the complex quadra-allelic SNP of mannose binding protein.
Nucleic Acid, Biomolecule And Polymer Identifier Codes
John BODEAU - San Mateo CA, US Heinz BREU - Palo Alto CA, US Kathleen PERRY - San Francisco CA, US Adam HARRIS - Carlsbad CA, US Patrick GILLES - Carlsbad CA, US Miho Gilles - Carsbad CA, US
Assignee:
LIFE TECHNOLOGIES CORPORATION - Carlsbad CA
International Classification:
C40B 30/04 C07H 21/04 C40B 40/06
US Classification:
506 9, 506 16, 536 2431
Abstract:
Provided herein are systems, compositions and methods for tracking, sorting and/or identifying sample polynucleotides using nucleic acid barcodes. The barcodes provided herein are oligonucleotides that are designed to be uniquely identifiable. The nucleic acid barcodes have properties that permit them to be sequenced with high accuracy and/or reduced error rates. In some embodiments, the nucleic acid barcodes are designed to have certain nucleotide sequences that make up overlapping dibase color positions (also called color positions). The order of the overlapping dibase color positions can be determined using fluorophore-encoded dibase probes in a fluorophore color calling scheme to give high fidelity reads.
Ucla Graduate School of Education and Information Studies Jan 2018 - Aug 2018
Undergraduate Research Associate | Equity and Access Studies In Education Project
Ucla Korean Culture Night Jan 2018 - Aug 2018
My wife I have one little boy named Brenden Patrick Gilles who is one. His brother Zackary Armand Gilles was born 02-05-09. Zack was born two months too soon and left this earth on ...