Paul E Cook

US Patent:

7777006, Aug 17, 2010

Filed:

Dec 31, 2002

Appl. No.:

10/334303

Inventors:

Scott M. Kee - Bourbonnais IL, US
Paul I. Cook - Kankakee IL, US
James R. Smith - Bourbonnais IL, US
Robert Kling - Bourbonnais IL, US
Scott A. Fowler - Indianapolis IN, US
David Weber - Bradley IL, US

Assignee:

CSL Behring L.L.C. - King of Prussia PA

International Classification:

A61K 35/14

US Classification:

530380, 530300, 530350, 435 71

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

8124736, Feb 28, 2012

Filed:

Aug 13, 2010

Appl. No.:

12/855721

Inventors:

Scott M Kee - Bourbonnais IL, US
Paul I. Cook - Kankakee IL, US
James R Smith - Bourbonnais IL, US
Robert Kling - Bourbonnais IL, US
Scott A Fowler - Apex NC, US
David Weber - Bourbonnais IL, US

Assignee:

CSL Behring L.L.C. - King of Prussia PA

International Classification:

A61K 35/14

US Classification:

530380, 530350, 530300

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

20120165261, Jun 28, 2012

Filed:

Feb 28, 2012

Appl. No.:

13/385642

Inventors:

Scott M. Kee - Bourbonnais IL, US
Paul I. Cook - Kankakee IL, US
James R. Smith - Bourbonnais IL, US
Robert Kling - Bourbonnais IL, US
Scott A. Fowler - Apex NC, US
David Weber - Bourbonnais IL, US

Assignee:

CSL BEHRING LLC - King of Prussia PA

International Classification:

A61K 38/17
A61P 11/00

US Classification:

514 153

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as a Cohn fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as a dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitant. Separation of the solid adsorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

40555240, Oct 25, 1977

Filed:

Aug 11, 1975

Appl. No.:

5/603771

Inventors:

Robert L. Colescott - Bourbonnais IL
Emil Kaiser - Chicago IL
Charles D. Bossinger - Olympia Fields IL
Paul I. Cook - Kankakee IL

Assignee:

Armour Pharmaceutical Company - Phoenix AZ

International Classification:

C08L 8900
C07C10352

US Classification:

260 8

Abstract:

Resin peptides useful in the preparation of peptides having biological activity, and particularly such resin peptides containing R-CH. sub. 2 -Phe-Glu at one end of an amino acid chain, R being the resin and Phe and Glu being the residues of the amino acids phenylalanine and glutamic acid; and processes for the preparation of such resin peptides. Resin peptides are disclosed which contain amino acid chains identical with the amino acid chains of natural peptides having biological activity. Other resin peptides are disclosed which contain amino acid chains in which the amino acid residues differ in kind and sequence from amino acid chains of natural biologically active peptides but from which peptides having biological activity may be derived.

US Patent:

20200078451, Mar 12, 2020

Filed:

May 14, 2019

Appl. No.:

16/411627

Inventors:

- King of Prussia PA, US
Paul I. COOK - Kankakee IL, US
James R. SMITH - Bourbonnais IL, US
Robert KLING - Bourbonnais IL, US
Scott A. FOWLER - Apex NC, US
David WEBER - Bradley IL, US

International Classification:

A61K 38/57
A61K 9/19
C07K 14/81
B01D 15/36
B01D 15/32

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as Coh fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitante. Spearation of the solid absorbent from the solution leaves a purified AAT solution that is directly suitabale for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

20180200347, Jul 19, 2018

Filed:

Mar 12, 2018

Appl. No.:

15/918614

Inventors:

- King of Prussia PA, US
Paul I. COOK - Kankakee IL, US
James R. SMITH - Bourbonnais IL, US
Robert KLING - Bourbonnais IL, US
Scott A. FOWLER - Apex NC, US
David WEBER - Bradley IL, US

International Classification:

A61K 38/57
B01D 15/36
C07K 14/81
A61K 9/19
B01D 15/32
G01N 30/46

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as Coh fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitante. Spearation of the solid absorbent from the solution leaves a purified AAT solution that is directly suitabale for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

20170042986, Feb 16, 2017

Filed:

Mar 18, 2016

Appl. No.:

15/074266

Inventors:

- King of Prussia PA, US
Paul I. COOK - Kankakee IL, US
James R. SMITH - Bourbonnais IL, US
Robert KLING - Bourbonnais IL, US
Scott A. FOWLER - Apex NC, US
David WEBER - Bradley IL, US

International Classification:

A61K 38/57
A61K 9/19
C07K 14/81

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as Coh fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitante. Spearation of the solid absorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.

US Patent:

20150320846, Nov 12, 2015

Filed:

Jul 17, 2015

Appl. No.:

14/802885

Inventors:

- King of Prussia PA, US
Paul I. COOK - Kankakee IL, US
James R. SMITH - Bourbonnais IL, US
Robert KLING - Bourbonnais IL, US
Scott A. FOWLER - Apex NC, US
David WEBER - Bourbonnais IL, US

International Classification:

A61K 38/57

Abstract:

A streamlined method for purifying alpha-1-antitrypsin (AAT) from an AAT-containing protein mixture, such as Coh fraction IV precipitate, is provided. In the method of the invention, contaminating proteins are destabilized by cleavage of disulfide bonds with a reducing reagent, such as dithiol, which does not affect AAT. The destabilized proteins are then preferentially adsorbed on a solid protein-adsorbing material, without the addition of a salt as a precipitante. Separation of the solid absorbent from the solution leaves a purified AAT solution that is directly suitable for chromatographic purification, without the need for extensive desalting as in prior art processes. A process incorporating this method, which provides pharmaceutical grade AAT in high yield on a commercial scale, is also described.