Paul Diaz

US Patent:

20060205026, Sep 14, 2006

Filed:

Dec 19, 2002

Appl. No.:

10/540277

Inventors:

Zhi Hong - Aliso Viejo CA, US
William Ricketts - Irvine CA, US
Paul Diaz - Riverside CA, US

Assignee:

Valeant Research & Development - Costa Mesa CA

International Classification:

C12Q 1/48
G06F 19/00

US Classification:

435015000, 702019000

Abstract:

A parallel inducible cell-based kinase screen (PICKS) includes a plurality of cells that express a kinase gene under the control of an inducer and a reporter gene, wherein the cells are derived from a single cell line, and wherein the reporter gene generate a signal in response to catalytic activity or inhibition of the expressed kinases. Particularly preferred systems include cells expressing kinases from a single kinase signaling pathway, kinases from a kinase family, and/or kinases from various different kinase signaling pathways. Consequently, contemplated systems provide a platform for screening for novel kinase inhibitors, inhibition specificity of particular kinase inhibitors, and for analysis of inter-pathway and/or intra-pathway inhibition of a kinase inhibitor.