Paul R Dunlap

US Patent:

57597987, Jun 2, 1998

Filed:

Dec 8, 1995

Appl. No.:

8/569973

Inventors:

Paul Vernon Dunlap - Woods Hole MA

Assignee:

Woods Hole Oceanographic Institution - Woods Hole MA

International Classification:

C12Q 102

US Classification:

435 29

Abstract:

The invention provides assays, kits and bacteria useful for detection of autoinducers. In a preferred aspect, the assay comprises 1) contacting a test sample suspected of containing an autoinducer with bacteria of the invention that are capable of producing an elevated amount of light in the presence of an exogenous autoinducer and that has at least two distinct genetic alterations that can each inhibit production of endogenous autoinducers; and 2) measuring the production of light. The sample will test positive for the presence of an autoinducer if a greater amount of light is produced relative to a control. The assays and kits have a variety of applications including use as an in vitro diagnostic for animal and plant disorders.

US Patent:

57861943, Jul 28, 1998

Filed:

Jan 5, 1995

Appl. No.:

8/369130

Inventors:

Paul Vernon Dunlap - Woods Hole MA

Assignee:

Woods Hole Oceanographic Institute - Woods Hole MA

International Classification:

C12N 916
C12N 1555

US Classification:

435196

Abstract:

Novel substantially pure periplasmic 3':5'-cyclic nucleotide phosphodiesterases are provided which are obtainable from gram negative bacterium capable of growing on restricted media containing cAMP or cGMP as a sole carbon source. Also provided is the isolated DNA coding for such enzymes and related methods of producing the same.

US Patent:

53825195, Jan 17, 1995

Filed:

Jan 13, 1993

Appl. No.:

8/004751

Inventors:

Paul V. Dunlap - Woods Hole MA
Sean M. Callahan - Woods Hole MA

Assignee:

Woods Hole Oceanographic Institution - Woods Hole MA

International Classification:

C12N 1531
C12N 1553
C12N 1570

US Classification:

435 691

Abstract:

Novel substantially pure periplasmic 3':5'-cyclic nucleotide phosphodiesterases are provided which are obtainable from gram negative bacterium capable of growing on restricted media containing cAMP or cGMP as a sole carbon source. Also provided is the isolated DNA coding for such enzymes and related methods of producing the same.