Paul C Ehrlich

US Patent:

47925280, Dec 20, 1988

Filed:

Feb 19, 1987

Appl. No.:

7/016213

Inventors:

Robert E. Canfield - New York NY
Paul H. Ehrlich - New York NY
William R. Moyle - Piscataway NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 33537
G01N 33561
G01N 33577

US Classification:

436515

Abstract:

Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

US Patent:

45145059, Apr 30, 1985

Filed:

May 21, 1982

Appl. No.:

6/380959

Inventors:

Robert E. Canfield - New York NY
Paul H. Ehrlich - New York NY
William R. Moyle - Piscataway NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 3354
G01N 3374
G01N 3376

US Classification:

436500

Abstract:

Mixtures of monoclonal antibodies which contain effective assaying amounts of each of at least two monoclonal antibodies that bind to different antigenic sites on the antigen and are capable under appropriate conditions of binding simultaneously to an antigen are useful in enhanced sensitivity assays for the antigen. By utilizing such mixtures in diagnostic assays for important antigens such as the polypeptide human chorionic gonadotropin enhanced sensitivity can be achieved as compared with assays employing individual monoclonal antibodies.

US Patent:

48513563, Jul 25, 1989

Filed:

Jul 14, 1987

Appl. No.:

7/072802

Inventors:

Robert E. Canfield - New York NY
Elmo G. Armstrong - Bronx NY
Paul H. Ehrlich - New York NY
Steven Birken - Dumont NJ

Assignee:

The Trustees of Columbia University in the City of New York - New York NY

International Classification:

G01N 3376
G01N 33535
G01N 33543

US Classification:

436510

Abstract:

An immunoassay for detecting and measuring hCG in a sample includes an antibody directed to the carboxy terminal portion of the. beta. subunit of hCG and a monoclonal antibody directed to a determinant on hCG at a locus sufficiently remote from the carboxy terminal portion of the. beta. subunit of hCG that both antibodies can simultaneously bind to hCG, wherein at least one of the antibodies is delectable when both are bound to hCG. In a presently preferred embodiment, an immunoassay for hCG or hCB. beta. in urine includes a purified, labeled or detectable serum-derived antibody directed to the carboxy-terminal portion of the. beta. subunit of hCG and a matrix-bound monoclonal antibody directed to a locus on the. beta. subunit sufficiently remote from the carboxy-terminal portion that both antibodies can simultaneously bind to hCG or hCG. beta.

US Patent:

43550231, Oct 19, 1982

Filed:

Sep 30, 1980

Appl. No.:

6/192696

Inventors:

Paul H. Ehrlich - White Plains NY
Gary R. Matsueda - Winchester MA
Michael N. Margolies - Weston MA
Edgar Haber - Weston MA

Assignee:

The Massachusetts General Hospital - Boston MA

International Classification:

A61K 39395

US Classification:

424 85

Abstract:

Immunologically active rabbit IgG antibody fragment compositions are produced by isolating the anti-light variable chain of rabbit IgG and/or the anti-heavy variable chain of rabbit IgG and combining them with each other or with the complementary heavy or light chain of rabbit IgG.