Paul S Nix

US Patent:

6402950, Jun 11, 2002

Filed:

Sep 20, 2000

Appl. No.:

09/665860

Inventors:

Paul T. Nix - Jackson NJ
Dennis R. Stocker - Yardley PA
Michael J. Byers - Spring Mills PA

Assignee:

Princeton Separations - Freehold NJ

International Classification:

B01D 3500

US Classification:

2103232, 210359, 210466, 210473, 210474, 210483, 422 72, 422101, 422102, 422104, 4353052, 436177, 436178

Abstract:

A device which can be used for filtering samples is described. The device is comprised of a plurality of filter wells, each having a wide region and a narrow region. The device is fitted with a filter element and then filled with a gel filtration matrix which is selected so that molecules of a particular molecular size will diffuse into the matrix. The device is centrifuged to remove water from the interstitial regions of the gel filtration matrix, and a sample is then added to the device containing the dried gel filtration matrix. The device is again centrifuged, causing smaller molecules to diffuse into the matrix and allowing larger molecules to exit the device, wherein they are collected and analyzed. The device is capable of filtering sample sizes as low as 1 L and can be used in a variety of existing laboratory equipment, including bench-top centrifuges and microcentrifuges. A process for using the device is also disclosed.

US Patent:

8124359, Feb 28, 2012

Filed:

Mar 16, 2007

Appl. No.:

12/294293

Inventors:

Stuart Levison - Edgewater NJ, US
Paul Nix - Jackson NJ, US
Derek Levison - Edgewater NJ, US

Assignee:

Aokin AG - Berlin

International Classification:

G01N 31/00
G01N 33/53

US Classification:

435 721, 435 4, 435 71, 4352879, 436501, 436518, 436525, 436529, 436535, 427287, 427337, 427338, 530300, 530350

Abstract:

A method for reducing non-specific binding in an assay is provided herein. The method includes (a) providing a reaction mixture, which includes or is suspected to include a first component and a second component capable of binding to each other in a specific binding reaction, and (b) adding non-physiological amounts of at least one additive to the reaction mixture before, during or after binding in a sufficient amount to reduce non-specific binding in the reaction mixture. The method further includes (c) monitoring or measuring the presence and/or concentration of at least one of the first and second components after step (b).

US Patent:

7993855, Aug 9, 2011

Filed:

Mar 16, 2007

Appl. No.:

12/294299

Inventors:

Stuart Levison - Edgewater NJ, US
Paul Nix - Jackson NJ, US
Derek Levison - Edgewater NJ, US

Assignee:

Aokin AG - Berlin

International Classification:

G01N 1/00
G01N 33/53

US Classification:

435 721, 435 2, 435 71, 436501, 436518, 436522, 422 50

Abstract:

A method of lowering the rate of a specific binding reaction in an assay for the detection and/or measurement of an analyte of interest is provided herein. In particular, the method includes providing a fluorescent conjugate of the analyte; a component capable of specifically binding to the analyte and its fluorescent conjugate; and a sample, which includes or is suspected to include the analyte. The method also includes allowing the specific binding component to interact simultaneously or at different times with the fluorescent conjugate of the analyte and the analyte in the sample, thereby forming a detectable complex due to the reaction between the fluorescent conjugate of the analyte and its specific binding component, wherein the reaction is performed in the presence of non-physiological amounts of at least one additive. The method further includes monitoring for the rate of change of the concentration of the detectable complex as a function of the amount of analyte in the sample.

US Patent:

20070042454, Feb 22, 2007

Filed:

Aug 17, 2005

Appl. No.:

11/206316

Inventors:

Paul Nix - Jackson NJ, US

International Classification:

C12Q 1/04
C12M 3/00

US Classification:

435034000, 435287100

Abstract:

A device, kit and method for detecting oral bacteria are provided. The device is a tube, which includes on its inside surface a coating of an agar medium selective for growing gram-positive bacteria. The device is particularly suitable for detecting the extent of growth of which provides an indication of the susceptibility of a patient to dental caries.

US Patent:

43048544, Dec 8, 1981

Filed:

Jul 1, 1980

Appl. No.:

6/165035

Inventors:

Paul T. Nix - Jackson NJ
Rebecca D. Goldfarb - Baltimore MD
Linda J. Stong - Hightstown NJ
Lorraine E. Sulick - Scotch Plains NJ
Ramesh C. Trivedi - Freehold NJ
Stanley W. Morgenstern - Neptune NJ

Assignee:

Worthington Biochemical Corporation - Freehold NJ

International Classification:

C12Q 154
C12Q 158
C12Q 140
C12P 1918

US Classification:

435 14

Abstract:

A maltodextrin phosphorylase limit dextrin in the presence of maltodextrin phosphorylase and inorganic phosphate, is used as a substrate for alpha-amylase, which initiates a series of enzymatic reactions resulting in a chromogen response which can be used to measure the concentration of alpha-amylase in a body fluid. A novel limit dextrin and its preparation also are described.

US Patent:

46736336, Jun 16, 1987

Filed:

Jun 28, 1984

Appl. No.:

6/625600

Inventors:

Thomas J. Kelleher - Springlake Heights NJ
Paul T. Nix - Jackson NJ

Assignee:

CooperBiomedical, Inc. - Malvern PA

International Classification:

G01N 100
G01N 140

US Classification:

435 4

Abstract:

A method for detecting the presence of cystic fibrosis ciliostatic factor in mammalian body fluid comprises contacting an enzyme whose activity is inhibited by the factor with a substrate for the enzyme in the presence of the body fluid, the substrate being substantially resistant to reactions catalyzed by enzymes in the body fluid, whereby the substrate is converted by the enzyme at a measurable rate, and comparing the rate of substrate conversion with the rate of substrate conversion by the enzyme in the absence of cystic fibrosis ciliostatic factor.

US Patent:

43213979, Mar 23, 1982

Filed:

Feb 20, 1980

Appl. No.:

6/123017

Inventors:

Paul T. Nix - Jackson NJ
Spencer M. Fields - Red Bank NJ

Assignee:

Millipore Corporation - Bedford MA

International Classification:

C07D23148
C07D23146

US Classification:

548366

Abstract:

A 3-aminobenzoic acid or a 3-aminobenzenesulfonic acid, when reacted with a 4-aminoantipyrine in aqueous medium in the presence of hydrogen peroxide forms a novel colored dye which is believed to be a charge-transfer complex of free radicals formed from the 3-aminoaromatic acid and the 4-aminoantipyrine. The dye and the process by which it is produced are useful in analytical procedures for hydrogen peroxide, as well as in enzymatic analyses involving the formation of hydrogen peroxide.

US Patent:

42476313, Jan 27, 1981

Filed:

Jan 31, 1979

Appl. No.:

6/008154

Inventors:

Paul T. Nix - Jackson NJ
Spencer Fields - Red Bank NJ

Assignee:

Millipore Corporation - Bedford MA

International Classification:

C12Q 162
C12Q 128

US Classification:

435 10

Abstract:

A 3-aminobenzoic acid or a 3-aminobenzenesulfonic acid, when reacted with a 4-aminoantipyrine in aqueous medium in the presence of hydrogen peroxide forms a novel colored dye which is believed to be a charge-transfer complex of free radicals formed from the 3-aminoaromatic acid and the 4-aminoantipyrine. The dye and the process by which it is produced are useful in analytical procedures for hydrogen peroxide, as well as in enzymatic analyses involving the formation of hydrogen peroxide.