Peter T Cheng

US Patent:

6495676, Dec 17, 2002

Filed:

Sep 3, 1999

Appl. No.:

09/390124

Inventors:

Michael L. Wood - Mountain View CA
David Albagli - Millbrae CA
Reuel B. Van Atta - Mountain View CA
Douglas Y. Thien - Menlo Park CA
Peter C. Cheng - San Jose CA
Bingfang Huan - Cupertino CA

Assignee:

Naxcor - Menlo Park CA

International Classification:

C07H 2100

US Classification:

536 253, 536 2531, 536 2537, 435 6

Abstract:

Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.

US Patent:

6573048, Jun 3, 2003

Filed:

Apr 18, 2000

Appl. No.:

09/551305

Inventors:

Reuel VanAtta - Mountain View CA
David Albagli - Menlo Park CA
Michael L. Wood - Mountain View CA
Peter C. Cheng - San Jose CA

Assignee:

Naxcor - Mountain View CA

International Classification:

C12Q 168

US Classification:

435 6, 435 71, 435 911, 435 912, 536 221, 536 231

Abstract:

Nucleic acid probes that are susceptible to chemical or enzymatic degradation are described herein. In addition, assays and methods using such probes in the detection of target nucleic acid sequences are disclosed. The target-specific hybridization region or target-complementary region of the degradable probes can be separated via a degradation process from the detectable region. The remaining portion of the degradable probes can be easily detected. The use of the degradable probes described herein improves the signal-to-noise ratio by reducing background specific or non-specific signal generation in assays and methods of nucleic acid detection.

US Patent:

6696246, Feb 24, 2004

Filed:

Aug 23, 1999

Appl. No.:

09/379888

Inventors:

Bingfang Huan - Cupertino CA
David Albagli - Menlo Park CA
Michael L. Wood - Mountian View CA
Reuel B. Van Atta - Mountain View CA
Peter C. Cheng - San Jose CA

Assignee:

Naxcor, Inc. - Menlo Park CA

International Classification:

C12Q 168

US Classification:

435 6, 435 911, 435 912, 536 221, 536 231, 536 243, 536 2431, 536 2432, 536 2433

Abstract:

Improved methods for in situ hybridization assays of cellular and subcellular systems and tissue sections, and immobilization-based assay techniques such as Northern blotting, Southern blotting, dot blots, and the like, and assay techniques wherein the probes are bound to substrates are disclosed. The subject invention employs crosslinker-containing hybridization probes capable of forming covalent bonds between the probe and the target nucleic acid. Upon activation, the crosslinker will, if the probe has hybridized with its essentially complementary target, form covalent bonds with the complementary strand to covalently crosslink the probe to the target. Subsequently, stringent wash conditions may be employed to reduce background signals due to non-specific absorption or probes or targets, while retaining all crosslinked probe/target hybrids. Also disclosed are diagnostic kits for use in clinical and diagnostic laboratories.

US Patent:

6737239, May 18, 2004

Filed:

Oct 15, 2002

Appl. No.:

10/272466

Inventors:

Michael L. Wood - Mountain View CA
David Albagli - Millbrae CA
Reuel B. Van Atta - Mountain View CA
Douglas Y. Thien - Menlo Park CA
Peter C. Cheng - San Jose CA
Bingfang Huan - Cupertino CA

Assignee:

Naxcor, Inc. - Menlo Park CA

International Classification:

C12Q 168

US Classification:

435 6, 536 243, 536 245, 514 44

Abstract:

Methods and compositions are provided for detecting nucleic acid sequences. Probes comprising a crosslinking agent are combined with a sample which may comprise a target sequence which is complementary to the probe. Hybridization is allowed to occur between complementary sequences. The crosslinking agent is activated. Covalent bonds are formed between the probe and the target sequence if they are hybridized to one another. The crosslinked nucleic acids can then be detected to indicate the presence of the target sequence. Also provided are kits comprising reagents.

US Patent:

6800768, Oct 5, 2004

Filed:

Sep 5, 2003

Appl. No.:

10/655021

Inventors:

Peter C. Cheng - San Jose CA
Tadashi J. Mizoguchi - Santa Clara CA

Assignee:

Naxcor, Inc. - Mountain View CA

International Classification:

C07D31114

US Classification:

549289, 549290

Abstract:

Novel coumarin derivatives comprising a coumarin moiety linked to a non-nucleosidic backbone moiety are disclosed. The resulting molecules are typically used as photoactivate crosslinking groups when incorporated into polynucleotides as replacements for one or more of the complementary nucleoside bases present in probes used in procedures involving nucleic acid hybridization reactions.

US Patent:

7052739, May 30, 2006

Filed:

May 9, 2003

Appl. No.:

10/434540

Inventors:

Gerardo Buitron - San Jose CA, US
Peter Cheng - Fremont CA, US
Walter Crofton - Castro Valley CA, US
Kwang Kon Kim - San Jose CA, US
William Maida - San Jose CA, US
David Newman - Fremont CA, US

Assignee:

Maxtor Corporation - Longmont CO

International Classification:

B05D 1/18

US Classification:

4274301, 118423, 118428, 118500

Abstract:

Various apparatus and methods are provided for lubricating hard memory disks. A mandrel is provided in one embodiment with three rows of teeth and provides a stable, three-point engagement with the inside edge of the disks at the center aperture. When removing disks from a cassette, the mandrel preferably places a uniform space between the disks. In a second embodiment, the mandrel may be used to lubricate single-sided hard memory disks. The single-sided hard memory disk may be positioned on the mandrel in pairs in a gap merge orientation, or equally spaced apart.

US Patent:

20040191806, Sep 30, 2004

Filed:

Dec 12, 2003

Appl. No.:

10/735174

Inventors:

Bingfang Huan - Cupertino CA, US
David Albagli - Menlo Park CA, US
Michael Wood - Mountain View CA, US
Reuel Van Atta - Mountain View CA, US
Peter Cheng - San Jose CA, US

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Improved methods for in situ hybridization assays of cellular and subcellular systems and tissue sections, and immobilization-based assay techniques such as Northern blotting, Southern blotting, dot blots, and the like, and assay techniques wherein the probes are bound to substrates are disclosed. The subject invention employs crosslinker-containing hybridization probes capable of forming covalent bonds between the probe and the target nucleic acid. Upon activation, the crosslinker will, if the probe has hybridized with its essentially complementary target, form covalent bonds with the complementary strand to covalently crosslink the probe to the target. Subsequently, stringent wash conditions may be employed to reduce background signals due to non-specific absorption or probes or targets, while retaining all crosslinked probe/target hybrids. Also disclosed are diagnostic kits for use in clinical and diagnostic laboratories.

US Patent:

20060115599, Jun 1, 2006

Filed:

Jan 12, 2006

Appl. No.:

11/332689

Inventors:

Gerardo Buitron - San Jose CA, US
Peter Cheng - Fremont CA, US
Walter Crofton - Castro Valley CA, US
Kwang Kim - San Jose CA, US
William Maida - San Jose CA, US
David Newman - Fremont CA, US

Assignee:

Maxtor Corporation - Longmont CO

International Classification:

B05D 1/18

US Classification:

427430100

Abstract:

Various apparatus and methods are provided for lubricating hard memory disks. A mandrel is provided in one embodiment with three rows of teeth and provides a stable, three-point engagement with the inside edge of the disks at the center aperture. When removing disks from a cassette, the mandrel preferably places a uniform space between the disks. In a second embodiment, the mandrel may be used to lubricate single-sided hard memory disks. The single-sided hard memory disk may be positioned on the mandrel in pairs in a gap merge orientation, or equally spaced apart.