Peter Yu Ma

US Patent:

6454796, Sep 24, 2002

Filed:

May 5, 2000

Appl. No.:

09/566234

Inventors:

Kimberley Barkman - San Mateo CA
Peter Ma - San Mateo CA
Richard S. Williams - Redwood City CA

Assignee:

Endovascular Technologies, Inc. - Menlo Park CA

International Classification:

A61F 206

US Classification:

623 135, 623 136

Abstract:

A bifurcated vascular graft having a tubular trunk with an inner diameter. Tubular limbs having respective inner diameters extend longitudinally from the trunk, and the diameter of at least one of the limbs is unequal to half the trunk diameter. In one embodiment, the graft is flared such that the combined diameters of the limbs exceed the diameter of the trunk. In another embodiment, the graft is tapered such that the diameter of the trunk exceeds the combined diameters of the limbs. In a third embodiment, the limb diameters are unequal to one another.

US Patent:

7407747, Aug 5, 2008

Filed:

Oct 15, 2002

Appl. No.:

10/272450

Inventors:

Kathleen M. Perry - San Francisco CA, US
Robert E. Ruhfel - San Francisco CA, US
Sandra L. Spurgeon - San Mateo CA, US
Peter Ma - Cupertino CA, US

Assignee:

Applera Corporation - Foster City CA

International Classification:

C12Q 1/68
C07H 21/00
C07H 21/02
C12P 19/34

US Classification:

435 6, 435 911, 435 912, 536 231, 536 253, 536 266

Abstract:

A dry composition comprising a nucleotide linked to a fluorescent dye is provided. A method of making a dry composition comprising the step of drying a solution comprising a nucleotide linked to a fluorescent dye is provided. A method is provided for performing a reaction on a nucleic acid substrate comprising: (a) providing a vessel containing a dried composition comprising a nucleotide linked to a fluorescent dye, and (b) adding to the vessel an aqueous solution and the nucleic acid substrate. A kit is provided for determining the sequence of a nucleic acid, the kit comprising a dry composition comprising a nucleotide linked to a fluorescent dye.

US Patent:

8486627, Jul 16, 2013

Filed:

Jan 7, 2010

Appl. No.:

12/684089

Inventors:

Peter Nien-Tung Ma - Cupertino CA, US

Assignee:

Applied Biosystems, LLC - Carlsbad CA

International Classification:

C12Q 1/68

US Classification:

435 61

Abstract:

In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.

US Patent:

20060141487, Jun 29, 2006

Filed:

Jan 26, 2005

Appl. No.:

11/044236

Inventors:

Peter Ma - Cupertino CA, US

International Classification:

C12Q 1/68
C12P 19/34

US Classification:

435006000, 435091200

Abstract:

In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.

US Patent:

20120135414, May 31, 2012

Filed:

Oct 28, 2011

Appl. No.:

13/284839

Inventors:

Linda LEE - Palo Alto CA, US
Sam Lee Woo - Redwood City CA, US
Peter Ma - Cupertino CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/00

US Classification:

435 612, 536 2433, 435 912

Abstract:

A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

US Patent:

20120270211, Oct 25, 2012

Filed:

Feb 15, 2012

Appl. No.:

13/397626

Inventors:

Linda LEE - Palo Alto CA, US
Sam WOO - Redwood City CA, US
Peter MA - Cupertino CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/68
C07H 21/04

US Classification:

435 61, 536 2433

Abstract:

A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.

US Patent:

20130344492, Dec 26, 2013

Filed:

Jun 12, 2013

Appl. No.:

13/916372

Inventors:

Peter MA - Cupertino CA, US

International Classification:

C12Q 1/68

US Classification:

435 612

Abstract:

In one aspect, there are provided methods of amplifying and sequencing a polynucleotide. In some embodiments, the method includes (a) amplifying the polynucleotide with at least one amplification primer, a processive amplification polymerase, a sequencing primer, a sequencing polymerase, deoxynucleoside triphosphates suitable for template-dependent primer extension, and one or more terminating nucleotides, the incubation being carried out at a first temperature suitable for amplifying the polynucleotide with the processive amplification polymerase; (b) incubating the product of step (a) at a second temperature suitable for forming a plurality of differently-sized extended sequencing primers with the sequencing polymerase; (c) evaluating the extended sequencing primers in order to determine the sequence of the polynucleotide. The reactions at the first and second temperatures can be carried out in a single reaction vessel. In other aspects, compositions and kits for carrying out the methods are also provided.

US Patent:

20210269871, Sep 2, 2021

Filed:

Mar 5, 2021

Appl. No.:

17/193232

Inventors:

- Carlsbad CA, US
Achim KARGER - Foster City CA, US
Peter MA - Cupertino CA, US
S. Jeffrey ROSNER - Palo Alto CA, US
Ian WALTON - Redwood City CA, US
Jonathan WANG - Mountain View CA, US
Michael WENZ - Redwood City CA, US

Assignee:

LIFE TECHNOLOGIES CORPORATION - Carlsbad CA

International Classification:

C12Q 1/6869
B01L 3/00
C12Q 1/6806

Abstract:

According to various embodiments described herein, a microfluidics-chip based purification device and system for Sanger-sequencing reactions is provided. The device and system allow for the introduction into a sequencing system of a cartridge containing purification technologies specific to the sequencing contaminants or sequencing method where the simplified purification solution of a cartridge allows automation of the sample purification process, reduced consumption of purification reagents, and consistency in sampling by reducing the sampling errors and artifacts. These various embodiments therefore solve the need for a microfluidics-chip-based, Sanger-sequencing reaction purification system for CE devices. The microfluidic chips described can be used as a PCR chip by reorganizing the on-chip reagents, reaction wells and work flow steps.