Kevin P. Campbell - Iowa City IA Derrick R. Witcher - Coralville IA Peter McPherson - Branford CT Steven D. Kahl - Fishers IN John D. Windass - Wokingham, GB Terence Lewis - Bracknell, GB Philip Bentley - Bracknell, GB
Assignee:
University of Iowa Research Foundation - Iowa City IA
International Classification:
G01N 33567 G01N 33534 C07K 1476
US Classification:
436504
Abstract:
Disclosed is a novel photo-activatable derivative of ryanodine 9-hydroxy-21-(4-azidobenzoyloxy)-9-epiryanodine. This novel ryanodine derivative has been conjugated to keyhole limpet hemocyanin (KLH) and used for the production of antibodies with high affinity and specificity to ryanodine. A radioimmunoassay specific for ryanodine was developed using the anti-ryanodine antibodies, and a dissociation constant for ryanodine of 1 nM was determined. Also disclosed is the binding of a labeled, photoactivatable derivative of ryanodine (ABRy) with the ryanodine receptor of skeletal, cardiac and brain membranes. Digestion of the labeled ryanodine receptor revealed a labeled 76 kDa tryptic fragment which is critical for the formation of the high affinity ryanodine binding site.
M. Peter McPherson (born October 27, 1940) was a special assistant to President Gerald Ford, head of USAID under President Ronald Reagan, president of ...