Philip M Lester

age ~66

from Oakland, CA

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Philip Lester Phones & Addresses

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Location:

United States

Work:

Genentech 2010 - 2010
Director

Separation Of Polypeptide Monomers
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US Patent:

6620918, Sep 16, 2003
Filed:

May 26, 1999
Appl. No.:

09/320100
Inventors:

Deborah Ann Ansaldi - San Mateo CA
Philip Lester - San Lorenzo CA
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

C07K 118
US Classification:

530416, 530412, 530413, 530417, 530418
Abstract:

A method is disclosed for separating a polypeptide monomer from a mixture comprising dimers and/or multimers. The method comprises applying the mixture to either a cation-exchange chromatography resin or an anion-exchange chromatography resin and eluting the mixture at a gradient of about 0-1 M of an elution salt, wherein the monomer is separated from the dimers and/or multimers present in the mixture.

Protein Purification
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US Patent:

6870034, Mar 22, 2005
Filed:

Feb 3, 2003
Appl. No.:

10/356974
Inventors:

Timothy N. Breece - San Francisco CA, US
Robert L. Fahrner - San Mateo CA, US
Jeffrey R. Gorrell - San Bruno CA, US
Kathlyn Pham Lazzareschi - San Mateo CA, US
Philip M. Lester - San Lorenzo CA, US
David Peng - Daly City CA, US
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

C07K001/22
US Classification:

530413, 436824, 436828
Abstract:

A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.

Purification Of Polypeptides
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US Patent:

7169908, Jan 30, 2007
Filed:

Jan 8, 2004
Appl. No.:

10/754212
Inventors:

Philip M. Lester - San Lorenzo CA, US
Josefine Persson - Foster City CA, US
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

C07K 1/02
C12P 21/02
US Classification:

530412, 530419, 435 691, 435 696
Abstract:

A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.

Purification Of Microbial Cell Fermentation Homogenates
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US Patent:

7579448, Aug 25, 2009
Filed:

Dec 12, 2006
Appl. No.:

11/609529
Inventors:

Philip M. Lester - San Lorenzo CA, US
Josefine Persson - Solna, SE
Assignee:

Genentech, Inc. - South San Francisco
International Classification:

C12P 21/02
C07D 219/00
A61K 39/395
US Classification:

530419, 530412, 435 696, 546102, 4241411
Abstract:

A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.

Separation Of Polypeptide Monomers
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US Patent:

20040116674, Jun 17, 2004
Filed:

Aug 15, 2003
Appl. No.:

10/642409
Inventors:

Deborah Ansaldi - San Mateo CA, US
Philip Lester - San Lorenzo CA, US
Assignee:

Genentech, Inc.
International Classification:

C07K016/28
US Classification:

530/363000, 530/387100, 530/388220
Abstract:

A method is disclosed for separating a polypeptide monomer from a mixture comprising dimers and/or multimers. The method comprises applying the mixture to either a cation-exchange chromatography resin or an anion-exchange chromatography resin and eluting the mixture at a gradient of about 0-1 M of an elution salt, wherein the monomer is separated from the dimers and/or multimers present in the mixture.

Protein Purification
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US Patent:

20050215769, Sep 29, 2005
Filed:

Mar 16, 2005
Appl. No.:

11/084729
Inventors:

Timothy Breece - San Francisco CA, US
Robert Fahrner - San Mateo CA, US
Jeffrey Gorrell - San Bruno CA, US
Kathlyn Lazzareschi - San Mateo CA, US
Philip Lester - San Lorenzo CA, US
David Peng - Daly City CA, US
International Classification:

C07K016/30
C07K016/24
US Classification:

530388220, 530416000, 530388230
Abstract:

A method for purifying proteins by Protein A chromatography is described which comprises removing contaminants by washing the solid phase with various intermediate wash buffers.

Aqueous Multiple-Phase Isolation Of Polypeptide
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US Patent:

57233101, Mar 3, 1998
Filed:

Feb 7, 1995
Appl. No.:

8/385187
Inventors:

Stuart Builder - Belmont CA
Roger Hart - Burlingame CA
Philip Lester - San Lorenzo CA
John Ogez - Redwood City CA
David Reifsnyder - San Mateo CA
Assignee:

Genentech, Inc. - South San Francisco CA
International Classification:

C07K 120
C07K 1465
C07K 1461
C12N 100
US Classification:

435 691
Abstract:

A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.

Refolding Of Polypeptides
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US Patent:

57566720, May 26, 1998
Filed:

Jun 6, 1995
Appl. No.:

8/470108
Inventors:

Stuart Builder - Belmont CA
Roger Hart - Burlingame CA
Philip Lester - San Lorenzo CA
David Reifsnyder - San Mateo CA
Assignee:

Genentech, Inc. - So. San Francisco CA
International Classification:

C07K 14475
A61K 3822
US Classification:

530350
Abstract:

A composition is provided comprising about 0. 1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0. 2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0. 1 to 9M of a chaotropic agent, and about 0. 01 to 15. mu. M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.