Ping Nie Lin

US Patent:

8569009, Oct 29, 2013

Filed:

Aug 20, 2007

Appl. No.:

11/841972

Inventors:

Ping Lin - San Diego CA, US
Andrea Ghetti - San Diego CA, US
Wenge Shi - San Marcos CA, US
Mengjia Tang - San Diego CA, US
Gioulnar I. Harvie - San Diego CA, US
Huimin Tao - San Diego CA, US
Guoliang Tao - San Diego CA, US
Lei Wu - San Diego CA, US
David Cerny - San Diego CA, US
Jia Xu - San Diego CA, US

Assignee:

Aviva Biosciences Corporation - San Diego CA

International Classification:

C12Q 1/24

US Classification:

435 30, 435 4, 435261, 436177

Abstract:

Provided are methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells, particularly including debulking that uses a microfabricated filter for filtering samples. The enriched rare cells can be used in a downstream process such as identification, characterization or growth in culture, or in other ways. Also included is a method of determining tumor aggressiveness or the number or proportion of cancer cells in the enriched sample by detecting telomerase activity, nucleic acid or expression after enrichment of rare cells. Also provided is an efficient, rapid method to specifically remove red and white blood cells from a biological sample containing at least one of the cell types, leading to enrichment of rare target cells including circulating tumor (CTC), stromal, mesenchymal, endothelial, fetal, stem, or non-hematopoietic cells et cetera from a blood sample.

US Patent:

20050095726, May 5, 2005

Filed:

Jul 14, 2004

Appl. No.:

10/892423

Inventors:

Xiangming Fang - San Diego CA, US
Lei Huang - San Diego CA, US
Ping Lin - San Diego CA, US
Jerald Feitelson - San Diego CA, US
Wei-Wei Zhang - San Diego CA, US

International Classification:

G01N033/543

US Classification:

436518000

Abstract:

Affinity separation compositions and methods are disclosed for separating targets from complex mixtures. Affinity reagents are bound to a solid support oriented in a manner to facilitate the activity of the affinity reagents which are capable of binding specific targets by affinity recognition. Affinity reagents include IgY antibodies, proteins, peptides, nucleotides and polymers. Targets include proteins, protein-protein complexes, protein-nucleotide complexes, nucleotides, cells and subcellular organelles.

US Patent:

20060252054, Nov 9, 2006

Filed:

Oct 31, 2005

Appl. No.:

11/264413

Inventors:

Ping Lin - San Diego CA, US
Douglas Yamanishi - Carlsbad CA, US
Paul Hujsak - San Diego CA, US
Sara Snyder - San Francisco CA, US
George Walker - San Diego CA, US
Mingxian Huang - San Diego CA, US
Guoliang Tao - San Diego CA, US
Lei Wu - San Diego CA, US
Xiaobo Wang - San Diego CA, US
Joe Ouyang - San Diego CA, US
Charina Schmitigal - San Diego CA, US
Samantha O'Neill - San Diego CA, US
Jing Cheng - San Diego CA, US
Elizabeth Voung - San Diego CA, US
Jia Xu - San Diego CA, US

International Classification:

C12Q 1/68
G01N 33/574

US Classification:

435006000, 435007230

Abstract:

The present invention recognizes that diagnosis and prognosis of many conditions can depend on the enrichment of rare cells, especially tumor cells, from a complex fluid sample such as a blood sample. In particular, the present invention is directed to methods and compositions for detecting a non-hematopoietic cell, e.g., a non-hematopoietic tumor cell, in a blood sample via, inter alia, removing red blood cells (RBCs) from a blood sample using a non-centrifugation procedure, removing white blood cells (WBCs) from said blood sample to enrich a non-hematopoietic cell, if any, from said blood sample; and assessing the presence, absence and/or amount of said enriched non-hematopoietic cell.

US Patent:

20080057505, Mar 6, 2008

Filed:

Jul 13, 2007

Appl. No.:

11/777962

Inventors:

Ping Lin - San Diego CA, US
Andrea Ghetti - San Diego CA, US
Wenge Shi - San Marcos CA, US
Mengjia Tang - San Diego CA, US
Gioulnar Harvie - San Diego CA, US
Huimin Tao - San Diego CA, US
Guoliang Tao - San Diego CA, US
Lei Wu - San Diego CA, US
David Cerny - San Diego CA, US
Jia Xu - San Diego CA, US

International Classification:

G01N 33/574
C12Q 1/02
C12Q 1/68
G01N 33/00

US Classification:

435006000, 435029000, 435007230, 435007240, 435007250

Abstract:

The present invention provides methods and compositions for isolating and detecting rare cells from a biological sample containing other types of cells. In particular, the present invention includes a debulking step that uses a microfabricated filters for filtering fluid samples and the enriched rare cells can be used in a downstream process such as identifies, characterizes or even grown in culture or used in other ways. The invention also include a method of determining the aggressiveness of the tumor or of the number or proportion of cancer cells in the enriched sample by detecting the presence or amount of telomerase activity or telomerase nucleic acid or telomerase expression after enrichment of rare cells. This invention further provides an efficient and rapid method to specifically remove red blood cells as well as white blood cells from a biological sample containing at least one of each of red blood cells and white blood cells, resulting in the enrichment of rare target cells including circulating tumor cells (CTC), stromal cells, mesenchymal cells, endothelial cells, fetal cells, stem cells, non-hematopoietic cells etc from a blood sample. The method is based upon combination of immuno-microparticles (antibody coated microparticles) and density-based separation. The final enriched target cells can be subjected to a variety of analysis and manipulations, such as flowcytometry, PCR, immunofluorescence, immunocytochemistry, image analysis, enzymatic assays, gene expression profiling analysis, efficacy tests of therapeutics, culturing of enriched rare cells, and therapeutic use of enriched rare cells. In addition, depleted plasma protein and white blood cells can be optionally recovered, and subjected to other analysis such as inflammation studies, gene expression profiling, etc.

US Patent:

20080176254, Jul 24, 2008

Filed:

Oct 18, 2007

Appl. No.:

11/874884

Inventors:

Xiangming Fang - San Diego CA, US
Lei Huang - San Diego CA, US
Ping Lin - San Diego CA, US
Jerald S. Feitelson - San Diego CA, US
Wei-Wei Zhang - San Diego CA, US

International Classification:

G01N 33/53
G01N 33/543
G01N 33/563
G01N 33/553
G01N 33/544
G01N 33/545

US Classification:

435 721, 436518, 436513, 436526, 436529, 436531

Abstract:

The present invention provides compositions and methods for affinity separation of targets from mixtures. In particular, disclosed are avian IgY antibodies coupled to solid supports and their methods of use.