Skills:
LC-MS Proficient in LC-MS/MS method development, hands on operations, trouble-shooting skills; triple quadrupole(QQQ) MS(AB Sciex), waters UPLC(Aquity), Leap/Agilent LC autosampler, Schimadzu pumps, Shimadzu prominence fluorescent detector, Symbiosis Hands on experience with reverse phase, ion exchange and normal phase chromatography (waters, phenomenex, ACE, thermo columns); mobile phase reagent selection, peak shape optimization, guard column selection; LC program development(isocratic, gradient, backflush, forward flush, diversion, peak focusing), multi charge MS tuning for large molecules under ESI mode, diastereomers and isomers resolution, etc. Experience with phospholipids monitoring, matrix effect, interference, and cross-talk. Sample Preparation Extensive experience in SPE, PPE, LLE sample preparation; experience with whole blood, Dry blood sopt (DBS) and tissue homogenization Experience with peptide and oligonucleotide sample preparation, fluorescent quantitation Experience with high through-put automated sample preparation using Tomtec, Hamilton automation system, Symbiosis online extraction Other skills and software Experience with UV-vis, FTIR, GC, NMR, EPR, PXRD, TGA and Wet Chemistry, TLC Experience with Analyst, Xcalibur, SmartLab, Watson-Lims, Symbiosis Pharma, Waters UPLC Acquity, Scienfinder, Kaleidagraph, Origin, Mercury, Mathcad and Chem Office, Microsoft Office