Rafael Dela Vega

US Patent:

7569340, Aug 4, 2009

Filed:

Aug 18, 2006

Appl. No.:

11/506200

Inventors:

Chad A. Mirkin - Wilmette IL, US
Rafael A. Vega - Evanston IL, US
Daniel Maspoch - Evanston IL, US
Khalid Salaita - Evanston IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C12Q 1/70
C12P 1/00
C12N 7/00
C12N 11/00

US Classification:

435 5, 435 41, 4352351, 977802

Abstract:

A nanoarray template utilizing coordination chemistry or metal ion binding to control the site-isolation and orientation of virus particles is provided. The nanoarray template is generated by lithography including Dip Pen Nanolithography. The surface chemistry that is inherent in many viruses, metal-ion based or inorganic coordination chemistry is used to immobilize individual virus particles without the need for their genetic modification. Single particle control enables a wide variety of studies involving viruses that are not possible with microarrays, including single particle, single cell infectivity studies, exploration of such structures as templates in materials synthesis and molecular electronics, and studies aimed at understanding how surface presentation influences their bioactivity. This is an example of such control at the single-particle level, and therefore, commercial use of nanoarrays in biological systems.

US Patent:

8318508, Nov 27, 2012

Filed:

Jun 17, 2008

Appl. No.:

12/213301

Inventors:

Chad A. Mirkin - Wilmette IL, US
Ling Huang - Corning NY, US
Rafael Vega - Evanston IL, US
Steven Lenhert - Karlsruhe, DE

Assignee:

Northwestern University - Evanston IL
Karlsruhe Institute of Technology - Karlsruhe

International Classification:

G01N 1/00

US Classification:

436180, 436 71

Abstract:

Better patterning methods, including for better methods for forming biomolecular arrays, including a method comprising: providing a tip and a substrate surface, disposing a patterning composition at the end of the tip, depositing at least some of the patterning composition from the tip to the substrate surface to form a deposit disposed on the substrate surface, wherein the patterning composition comprises at least one lipid, optionally at least one solvent, and at least one patterning species different from the lipid and the optional solvent. The lipid can be a phospholipid such as DOPC. The patterning species can be an oligonucleotide or a protein. Microarrays and nanoarrays can be prepared including nanoscale resolution of deposits. The lipid can activate patterning or increase the rate of patterning. Simplified tip preparation can be achieved.

US Patent:

20100160182, Jun 24, 2010

Filed:

Jun 13, 2006

Appl. No.:

11/917415

Inventors:

Chad A. Mirkin - Wilmette IL, US
Rafael A. Vega - Evanston IL, US
Daniel Maspoch - Bellaterra, ES
Clifton Kwang-Fu Shen - Evanston IL, US
Joseph J. Kakkassery - Evanston IL, US

Assignee:

Northwestern University - Evanston IL

International Classification:

C40B 40/00
C40B 50/18
C40B 40/10
C40B 40/12

US Classification:

506 18, 506 32, 506 13, 506 19

Abstract:

A method for immobilizing unmodified material using a metal-ion approach is provided wherein the material is immobilized on a surface in an active state on surface features coupled with metal-ions.

US Patent:

20110059431, Mar 10, 2011

Filed:

Jun 18, 2008

Appl. No.:

12/665326

Inventors:

Chad A. Mirkin - Wilmette IL, US
Rafael A. Vega - Evanston IL, US
Haley D. Hill - Evanston IL, US

Assignee:

NORTHWESTERN UNIVERSITY - Evanston IL

International Classification:

C12Q 1/70
C12Q 1/68

US Classification:

435 5, 435 6

Abstract:

The present invention relates to methods for detecting for the presence of one or more target analytes, e.g. biomolecules, in a sample. In particular, the present invention relates to a method that utilizes blocking strands to inhibit target rehybridization to detect double stranded genomic DNA.