Rajesh Kumar Gaur

US Patent:

RE43000, Dec 6, 2011

Filed:

May 6, 2010

Appl. No.:

12/775422

Inventors:

Rajesh K. Gaur - Glendora CA, US
Sailesh Gopalakrishna Pillai - Azusa CA, US

Assignee:

City of Hope - Duarte CA

International Classification:

C12Q 1/68
C12P 19/34
C07H 21/02
C07H 21/04

US Classification:

435 911, 435 6, 435 9131, 536 231

Abstract:

The present invention relates to riboswitches that have been engineered to regulate pre-mRNA splicing. In particular, the insertion of a high affinity theophylline binding aptamer into the 3′ splice site region, 5′ splice site region, or branchpoint sequence (BPS) of a pre-mRNA modulates RNA splicing in the presence of theophylline. Accordingly, the aspects of the present invention include, but are not limited to, theophylline-dependent riboswitches which modulate RNA splicing, methods of modulating RNA splicing using theophylline and its corresponding riboswitches, methods of improving/identifying theophylline-dependent riboswitches, methods of treating diseases associated with or caused by abnormal RNA splicing.

US Patent:

20090170793, Jul 2, 2009

Filed:

Jun 1, 2006

Appl. No.:

11/446527

Inventors:

Rajesh K. Gaur - Glendora CA, US

International Classification:

A61K 31/7088
C07H 21/04
C12N 15/74
C12N 5/00
C07H 21/00
G01N 33/566
A61P 43/00

US Classification:

514 44, 536 241, 4353201, 435325, 536 254, 436501

Abstract:

The present invention relates to riboswitches that have been engineered to regulate pre-mRNA splicing. In particular, the insertion of a high affinity theophylline binding aptamer into the 3′ splice site region, 5′ splice site region, or branchpoint sequence (BPS) of a pre-mRNA modulates RNA splicing in the presence of theophylline. Accordingly, the aspects of the present invention include, but are not limited to, theophylline-dependent riboswitches which modulate RNA splicing, methods of modulating RNA splicing using theophylline and its corresponding riboswitches, methods of improving/identifying theophylline-dependent riboswitches, methods of treating diseases associated with or caused by abnormal RNA splicing.