Ralph E Dewey

US Patent:

7884263, Feb 8, 2011

Filed:

Oct 13, 2006

Appl. No.:

11/580765

Inventors:

Ralph E. Dewey - Apex NC, US
Steven W. Bowen - Raleigh NC, US
Balazs Siminszky - Lexington KY, US
Lily Gavilano - Lexington KY, US

Assignee:

North Carolina State University - Raleigh NC
University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 15/82

US Classification:

800285, 800287

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

8124851, Feb 28, 2012

Filed:

Nov 12, 2008

Appl. No.:

12/269531

Inventors:

Ralph E. Dewey - Apex NC, US
Balazs Siminszky - Neuchatel, CH
Steven W. Bowen - Raleigh NC, US
Lily Gavilano - Raleigh NC, US

Assignee:

North Carolina State University - Raleigh NC
University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 15/82
C12N 15/29

US Classification:

8003173, 536 245

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

20110174322, Jul 21, 2011

Filed:

Dec 17, 2010

Appl. No.:

12/971801

Inventors:

Ralph E. Dewey - Apex NC, US
Steven W. Bowen - Raleigh NC, US
Balazs Siminszky - Lexington KY, US
Lily Gavilano - Lexington KY, US

Assignee:

North Carolina State University - Raleigh NC
University of Kentucky Research Foundation - Lexington KY

International Classification:

C12N 15/87
C07H 21/04
C12N 15/63
A01H 5/00
C07K 14/415
C12Q 1/68
A24B 15/10

US Classification:

131280, 800285, 536 231, 4353201, 8003173, 530370, 435 61, 536 232, 800278, 131111, 131290

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

20120117933, May 17, 2012

Filed:

Jan 30, 2012

Appl. No.:

13/361159

Inventors:

Ralph E. Dewey - Apex NC, US
Balazs Siminszky - Neuchatel, CH
Steven W. Bowen - Raleigh NC, US
Lily Gavilano - Raleigh NC, US

Assignee:

University of Kentucky Research Foundation - Lexington KY
North Carolina State University - Raleigh NC

International Classification:

A01D 45/16
C12N 15/82
A01H 5/10
A01H 1/06
A01H 5/00

US Classification:

56 275, 800276, 8003173, 800278

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

20120118308, May 17, 2012

Filed:

Jan 30, 2012

Appl. No.:

13/361235

Inventors:

Ralph E. Dewey - Apex NC, US
Balazs Siminszky - Neuchatel, CH
Steven W. Bowen - Raleigh NC, US
Lily Gavilano - Raleigh NC, US

Assignee:

University of Kentucky Research Foundation - Lexington KY
North Carolina State University - Raleigh NC

International Classification:

A24B 15/18
C07H 21/04
C12N 15/63
C12Q 1/68
A01H 5/00
A01H 5/10
C12N 5/10
C12N 15/82
A24D 1/00
C12N 9/14

US Classification:

131331, 131352, 536 232, 4353201, 435195, 8003173, 435414, 800278, 435 618, 435 612

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

20120216822, Aug 30, 2012

Filed:

Feb 28, 2012

Appl. No.:

13/407421

Inventors:

Ramsey S. Lewis - Apex NC, US
Ralph E. Dewey - Apex NC, US

International Classification:

A24B 13/00
A24B 13/02
A01H 5/12
A24D 1/04
A01H 5/00
A01H 5/10

US Classification:

131331, 8003173, 131352

Abstract:

The present invention provides tobacco inbred plants NCBEX1F and NCBEX1MS, and NC EX90. The present invention also provides parts of such plants and products made from those parts. The present invention also includes progeny of the provided plants including hybrids.

US Patent:

20130037040, Feb 14, 2013

Filed:

Jan 13, 2011

Appl. No.:

13/521766

Inventors:

Ralph E. Dewey - Apex NC, US
Ramsey S. Lewis - Apex NC, US

Assignee:

NORTH CAROLINA STATE UNIVERSITY - Raleigh NC

International Classification:

A01H 5/00
C12N 9/18
A24B 15/18
C12N 15/82
A01H 5/10
C12Q 1/68
A01H 1/02

US Classification:

131290, 435 618, 435197, 536 232, 800260, 800278, 8003173, 131352

Abstract:

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in tobacco plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for a root-specific nicotine demethylases, CYP82E10, and variants thereof, that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Compositions of the invention also include tobacco plants, or plant parts thereof, comprising a mutation in a gene encoding a CYP82E10 nicotine demethylase, wherein the mutation results in reduced expression or function of the CYP82E10 nicotine demethylase. Seed of these tobacco plants, or progeny thereof, and tobacco products prepared from the tobacco plants of the invention, or from plant parts or progeny thereof, are also provided. Methods for reducing the level of nornicotine, or reducing the rate of conversion of nicotine to nornicotine, in a tobacco plant, or plant part thereof are also provided. The methods comprise introducing into the genome of a tobacco plant a mutation within at least one allele of each of at least three nicotine demethylase genes, wherein the mutation reduces expression of the nicotine demethylase gene, and wherein a first of these nicotine demethylase genes encodes a root-specific nicotine demethylase involved in the metabolic conversion of nicotine to nornicotine in a tobacco plant or a plant part thereof. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

US Patent:

20130091606, Apr 11, 2013

Filed:

Jun 22, 2012

Appl. No.:

13/531424

Inventors:

Wanjun Zhang - Beijing, CN
Rongda Qu - Holly Springs NC, US
Ralph E. Dewey - Apex NC, US

Assignee:

BAYER CROPSCIENCE LP - Research Triangle Park NC

International Classification:

C12N 15/82

US Classification:

800294, 435431

Abstract:

The present invention relates to a method of -based transformation of a plant comprising: generating a callus explant by, performing callus induction and maintenance in a plant in at least a first solution comprising less than about 100 mg/l of inositol; subjecting the callus explant to a cold treatment in a second solution comprising glutamine; and co-cultivating the callus explant with an in a third solution comprising glutamine. The present invention also relates to a callus induction medium and/or callus maintenance medium, comprising: less than about 100 mg/l of inositol, and optionally at least one constituent selected from the group consisting of: an N6 salt or any plant culture media, proline, casein hydrolysate, a B-Vitamin, maltose, phytagel, 2,4-D, BAP and/or other plant growth regulators/hormones.