Randor R Radakovits

US Patent:

20130102040, Apr 25, 2013

Filed:

Oct 17, 2012

Appl. No.:

13/654347

Inventors:

Randor Radakovits - Denver CO, US
Robert Jinkerson - Golden CO, US
Matthew Posewitz - Golden CO, US

Assignee:

COLORADO SCHOOL OF MINES - Golden CO

International Classification:

C12N 15/79

US Classification:

435134, 4353201, 4352572, 435471, 536 241, 536 237

Abstract:

The present disclosure is directed to novel polynucleotide sequences for use in . The novel polynucleotide sequences include control sequences and coding sequences. Also disclosed are novel gene expression constructs wherein promoters/control regions are operatively linked to or non-coding sequences. These novel polynucleotide sequences and expression constructs can be introduced into and can recombine into the genome. Expression from these polynucleotide sequences and expression constructs can enhance biomass and/or lipid biosynthesis. Also disclosed are methods for modifying , for example by stably transforming with nucleic acid sequences, growing the modified , and obtaining biomass and biofuels from the modified

US Patent:

20120329099, Dec 27, 2012

Filed:

Mar 26, 2012

Appl. No.:

13/430383

Inventors:

Matthew C. Posewitz - Golden CO, US
Randor Radakovits - Denver CO, US
Robert Jinkerson - Golden CO, US
Victoria H. Work - Golden CO, US
Jonathan E. Meuser - La Jolla CA, US

Assignee:

Colorado School of Mines - Golden CO

International Classification:

C12N 1/13
C12P 19/14

US Classification:

435 99, 4352572

Abstract:

Methods, processes, and systems for the production of lipids and starch from modified algae are disclosed. In one embodiment, the modified algae over-expresses isoamylase and accumulates much higher amounts of starch than unmodified algae. In some embodiments, the modified algae comprises one or more copies of an isoamylase expression construct. In one embodiment, the modified algae is a sta7 mutant with a starchless phenotype that has been complemented with one or more copies of the wild-type genomic STA7 isoamylase gene construct. The complemented, modified algae accumulates much greater amount of starch than an unmodified algae and may be used to produce large amounts of starch and/or lipids.

US Patent:

20190203221, Jul 4, 2019

Filed:

Dec 27, 2018

Appl. No.:

16/234209

Inventors:

- La Jolla CA, US
Fedor I. Kuzminov - La Mesa CA, US
Randor R. Radakovits - Poway CA, US
John H. Verruto - San Diego CA, US
Sarah Tacke - San Diego CA, US
Roberto Spreafico - La Jolla CA, US
William F. Lambert - San Diego CA, US
Jessica Nichole Greiner - San Diego CA, US

International Classification:

C12N 15/82
C12N 15/62
A61K 36/02
C12N 15/10

Abstract:

Mutant photosynthetic organisms having reduced chlorophyll and increased photosynthetic efficiency are provided. The mutant strains have mutated or attenuated: chloroplastic SRP54 gene and SGI1 gene; chloroplastic SRP54 gene and SGI2 gene; chloroplastic SRP54 gene, SGI1, and SGI2 genes are disclosed. The mutant photosynthetic organisms exhibit increased productivity with respect to wild-type strains. Also provided are mutant photosynthetic organisms having mutated or attenuated cytosolic SRP54 genes. Provided herein are methods of producing biomass and other products such as lipids using strains having mutations in an SRP54 gene, SGI1, SGI2 genes, a combination of SGI1/SRP54, and a combination of SGI2 and SRP54 genes. Also included are constructs and methods for attenuating or disrupting SRP54, SGI1, and SGI2 genes.

US Patent:

20180186842, Jul 5, 2018

Filed:

Dec 29, 2017

Appl. No.:

15/859094

Inventors:

- La Jolla CA, US
Nicholas Bauman - San Diego CA, US
Randor R. Radakovits - Poway CA, US
Roberto Spreafico - San Diego CA, US
Fedor Kuzminov - San Diego CA, US
Imad Ajjawi - San Diego CA, US
Saheed Imam - San Diego CA, US
Andrew Schultz - San Diego CA, US
Kathleen Kwok - San Diego CA, US
Moena Aqui - San Diego CA, US
Jennifer Nominati - San Diego CA, US
John Verruto - San Diego CA, US
Shaun Bailey - San Diego CA, US

International Classification:

C07K 14/405
C12N 1/36
C12N 1/12
C12P 7/64
C12P 21/00
C12N 15/01
C12N 15/82

Abstract:

Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.

US Patent:

20160264985, Sep 15, 2016

Filed:

Mar 11, 2016

Appl. No.:

15/068448

Inventors:

- La Jolla CA, US
Elizabeth A. Felnagle - Seattle WA, US
Jun Urano - Irvine CA, US
Maung N. Win - San Diego CA, US
Randor R. Radakovits - Escondido CA, US

International Classification:

C12N 15/82
C12N 9/00
C12N 9/02

Abstract:

Recombinant microorganisms engineered for the production of polyunsaturated fatty acids (PUFAs) are provided. Also provided are biomass, microbial oils, and food products and ingredients produced by or comprising the microorganisms of the invention.