Rich M Meyer

US Patent:

8436025, May 7, 2013

Filed:

Sep 17, 2009

Appl. No.:

12/561750

Inventors:

Janet Ajioka - Seattle WA, US
Heidi S. Hummel - Seattle WA, US
Rich B. Meyer - San Mateo CA, US
John Swindle - Seattle WA, US

Assignee:

CompleGen Partners, Inc. - Seattle WA

International Classification:

A61K 31/136
A61K 31/4412
A61P 29/00
A61P 37/06
A61P 3/10

US Classification:

514349, 514646

Abstract:

The present invention provides a method of selectively inhibiting PKCθ in the presence of PKCδ, by administering to a subject in need thereof, a therapeutically effective amount of a compound of Formula I. The present invention also provides a method of inhibiting cytokine synthesis in a T cell, a method of inhibiting T cell proliferation, and a method of inhibiting the replication of and cytokine production by T lymphocytes, while not stimulating or inhibiting the replication of B lymphocytes.

US Patent:

20020052482, May 2, 2002

Filed:

Dec 18, 2000

Appl. No.:

09/739928

Inventors:

Igor Kutyavin - Bothell WA, US
Eugeny Lukhtanov - Bothell WA, US
Howard Gamper - Woodinville WA, US
Rich Meyer - Woodinville WA, US

Assignee:

Microprobe

International Classification:

C07H021/02
C07H021/04

US Classification:

536/023100, 536/024300

Abstract:

Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strogly bind to the target sequence of the complementary strand.

US Patent:

20020146690, Oct 10, 2002

Filed:

Nov 1, 1999

Appl. No.:

09/431385

Inventors:

RICH MEYER - BOTHELL WA, US
IRINA AFONINA - MILL CREEK WA, US
IGOR KUTYAVIN - BOTHELL WA, US

International Classification:

C12Q001/68
C07H021/00

US Classification:

435/006000, 536/023100, 536/024300

Abstract:

Oligonucleotides in which one or more purine residues are substituted by pyrazolo[3,4-d]pyrimidines exhibit improved hybridization properties. Oligonucleotides containing pyrazolo[3,4-d]pyrimidine base analogues have higher melting temperatures than unsubstituted oligonucleotides of identical sequence. Thus, in assays involving hybridization of an oligonucleotide probe to a target polynucleotide sequence, higher signals are obtained. In addition, mismatch discrimination is enhanced when pyrazolo[3,4-d]pyrimidine-containing oligonucleotides are used as hybridization probes, making them useful as probes and primers for hybridization, amplification and sequencing procedures, particularly those in which single- or multiple-nucleotide mismatch discrimination is required.

US Patent:

20030143602, Jul 31, 2003

Filed:

Nov 22, 2002

Appl. No.:

10/302608

Inventors:

Rich Meyer - Bothell WA, US
Irina Afonina - Mill Creek WA, US
Igor Kutyavin - Bothell WA, US

International Classification:

C12Q001/68
C07H021/04

US Classification:

435/006000, 536/024300

Abstract:

Oligonucleotides in which one or more purine residues are substituted by pyrazolo[3,4-d]pyrimidines exhibit improved hybridization properties. Oligonucleotides containing pyrazolo[3,4-d]pyrimidine base analogues have higher melting temperatures than unsubstituted oligonucleotides of identical sequence. Thus, in assays involving hybridization of an oligonucleotide probe to a target polynucleotide sequence, higher signals are obtained. In addition, mismatch discrimination is enhanced when pyrazolo[3,4-d]pyrimidine-containing oligonucleotides are used as hybridization probes, making them useful as probes and primers for hybridization, amplification and sequencing procedures, particularly those in which single- or multiple-nucleotide mismatch discrimination is required.

US Patent:

20040058322, Mar 25, 2004

Filed:

Apr 22, 2002

Appl. No.:

10/127936

Inventors:

Joel Hedgpeth - San Francisco CA, US
Irina Afonina - Mill Creek WA, US
Igor Kutyavin - Bothell WA, US
Eugeny Lukhtanov - Bothell WA, US
Evgeniy Belousov - Mill Creek WA, US
Rich Meyer - Bothell WA, US

Assignee:

Epoch Pharmaceuticals, Inc. - Bothell WA

International Classification:

C12Q001/68

US Classification:

435/006000

Abstract:

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPIgroup resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

US Patent:

20050064463, Mar 24, 2005

Filed:

Jun 16, 2004

Appl. No.:

10/870578

Inventors:

Joel Hedgpeth - San Francisco CA, US
Irina Afonina - Mill Creek WA, US
Igor Kutyavin - Bothell WA, US
Eugeny Lukhtanov - Bothell WA, US
Evgeniy Belousov - Mill Creek WA, US
Rich Meyer - Bothell WA, US

International Classification:

C12Q001/68
C07H021/04
C12P019/34

US Classification:

435006000, 435091200, 536024300

Abstract:

Conjugates between a minor groove binding molecule, such as the trimer of 1,2-dihydro-(3H)-pyrrolo[3,2-e]indole-7-carboxylate (CDPI), and an oligonucleotide form unusually stable hybrids with complementary target sequences, in which the tethered CDPIgroup resides in the minor groove of the duplex. These conjugates can be used as probes and primers. Due to their unusually high binding affinity, conjugates as short as 8-mers can be used as amplification primers with high specificity and efficiency. MGB conjugation also increases the discriminatory power of short oligonucleotides, providing enhanced detection of nucleotide sequence mismatches by short oligonucleotides. The MGB-conjugated probes and primers described herein facilitate various analytic and diagnostic procedures, such as amplification reactions, PCR, detection of single-nucleotide polymorphisms, gene hunting, differential display, fluorescence energy transfer, hydrolyzable probe assays and others; by allowing the use of shorter oligonucleotides, which have higher specificity and better discriminatory power.

US Patent:

56523599, Jul 29, 1997

Filed:

Dec 2, 1993

Appl. No.:

8/162590

Inventors:

Rich B. Meyer - Woodinville WA
Alexander A. Gall - Bothell WA
Arthur D. Broom - Salt Lake City UT

Assignee:

Epoch Pharmaceuticals, Inc. - Washington DC

International Classification:

C07H 2102
A61K 3170

US Classification:

536 255

Abstract:

Oligonucleotides containing 1-N-alkyl-6-thiopurine, 3-N-alkyl-4-thiopyrimidine and 5-N-alkyl-4-thiopyrazolopyrimidine bases and the corresponding 2'-O-alkylated or allylated nucleotides demonstrate potent antiviral activity in several assays, including the human immunodeficiency virus reverse transcriptase enzyme assay. The oligonucleotides of the invention contain approximately 5 to 99 nucleotide units, and may include, in addition to the above-noted N-alkylated and thiolated heterocyclic bases, the naturally occurring pyrimidine and purine bases.

US Patent:

60841028, Jul 4, 2000

Filed:

Aug 27, 1998

Appl. No.:

9/141764

Inventors:

Igor V. Kutyavin - Bothell WA
Eugeny A. Lukhtanov - Bothell WA
Howard B. Gamper - Woodinville WA
Rich B. Meyer - Woodinville WA

Assignee:

Epoch Pharmaceuticals, Inc. - Redmond WA

International Classification:

C07D29300
C07D41700
C07H 2100
A01N 4304

US Classification:

548100

Abstract:

Minor groove binding molecules are covalently bound to oligonucleotides which in their base sequence are complementary to a target sequence of single stranded or double stranded DNA, RNA or hybrids thereof. The covalently bound oligonucleotide minor groove binder conjugates strongly bind to the target sequence of the complementary strand.