Richard A Carroll

US Patent:

46407855, Feb 3, 1987

Filed:

Dec 24, 1984

Appl. No.:

6/685801

Inventors:

Richard J. Carroll - Syracuse NY
Albert A. Luderer - Marshfield MA
Ward C. Smith - Painted Post NY
Anthony R. Zine - Corning NY

Assignee:

Becton Dickinson and Company - Paramus NJ

International Classification:

B01D 2126

US Classification:

210782

Abstract:

This invention is directed to means for inhibiting the apparent shift in the buoyant density of and/or to restore any loss in the buoyant density of the granulocytic white blood cells in a sample of blood, thereby insuring the quality of the separation of lymphocytes and monocytes from granulocytes in a blood sample. The invention comprises contacting a blood sample with a hypertonic fluid containing a low molecular weight organic or inorganic ionic substance and/or with an isotonic or hypertonic fluid containing a high molecular weight organic substance which may contain organic molecules having a lipophilic substituent in their structures and/or with a culture medium for blood cells. An integral part of the invention is an improved blood separation tube utilizing a gel-like substance having a specific gravity between 1. 060-1. 065 g/cm. sup. 3 to significantly enhance the purity of cell separation, while providing acceptable cell yields.

US Patent:

59067446, May 25, 1999

Filed:

Sep 9, 1997

Appl. No.:

8/925851

Inventors:

Richard J. Carroll - Syracuse NY
Frank A. Augello - Cedar Knolls NJ

Assignee:

Becton Dickinson and Company - Franklin Lakes NJ

International Classification:

B01D 2126

US Classification:

210516

Abstract:

A collection device for plasma preparation for diagnostic assays. The device comprises a spray-dried anticoagulant formulation on the interior surface of the device and a thixotropic polymeric gel. The device is an improvement over commercially available devices which contain liquid anticoagulant formulations, for use in nucleic acid testing that employ amplifications technologies including, but not limited to, polymerase chain reaction (PCR), branched DNA (bDNA) and nucleic acid sequenced based amplification (NASBA).